Expression of placental protein 11 in oral squamous cell carcinoma and its clinical significance

doi:10.19438/j.cjoms.2025.04.006

Abstract

Abstract: PURPOSE: To investigate the expression and clinical significance of placental protein 11 (ENDOU) in oral squamous cell carcinoma(OSCC). METHODS: The expression of ENDOU in tumor tissues and normal tissues was comparatively analyzed through the TCGA database, and the correlation between ENDOU expression and clinical parameters was explored. Subsequently, the expression of ENDOU in 20 cases of primary OSCC and corresponding adjacent tissues was detected by immunohistochemical staining. The ENDOU overexpressing human OSCC cell line SCC-25 was constructed. Furthermore, the effects of ENDOU on the proliferation, apoptosis and invasion of OSCC cells were evaluated by CCK-8 assay, flow cytometry apoptosis assay, and Transwell cell invasion assay. RESULTS: Bioanalytical results indicated that the expression of the ENDOU gene in OSCC was significantly lower than that in adjacent tissues(P<0.05); there was significant difference in the expression of the ENDOU gene among different T stages and clinical stages of primary tumors(P<0.05), and the expression was significantly lower in cervical lymph node metastasis group than in the non-metastasis group(P<0.05). Immunohistochemical experiments showed that the expression level of ENDOU in OSCC tissues was significantly lower than that in adjacent tissues (P<0.05); overexpression of ENDOU could inhibit the proliferation and migration of oral squamous cell carcinoma cells in vitro. CONCLUSIONS: Downregulation of EDNOU expression in OSCC may play an important role in tumor formation and development, and is expected to serve as a tumor biomarker for OSCC.

Key words: Placental protein 11, Oral squamous cell carcinoma, Proliferation, Invasion, Apoptosis

CLC Number:

R739.8

Alafati Heyasiding, An Wei, Janaer Tuergenbieke, Jin Xuemei, Guo Sha, Yao Zhitao. Expression of placental protein 11 in oral squamous cell carcinoma and its clinical significance[J]. China Journal of Oral and Maxillofacial Surgery, 2025, 23(4): 347-354.

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