The inhibitory effect of antibacterial peptides on osteoblast apoptosis induced by TNF-α

doi:10.19438/j.cjoms.2025.04.003

Abstract

Abstract: PURPOSE: To explore the inhibitory effect of antimicrobial peptides on osteoblast apoptosis induced by tumor necrosis factor-α(TNF-α). METHODS: Osteoblasts were divided into MEM control group, antimicrobial peptide group, TNF-α group, and TNF-α+ antimicrobial peptide group. After 1 day of osteoblast culture, the antimicrobial peptide group was added with a final concentration of 20 μg/mL of antimicrobial peptide. The TNF-α group and the TNF-α + antimicrobial peptide group were induced with 50 ng/mL TNF-α for 1 day. Then, the TNF-α + antimicrobial peptide group was added with a final concentration of 20 μg/mL of antimicrobial peptide and continued to be cultured for 1 day. Apoptosis of cells was detected by flow cytometry. Cell migration ability was detected by scratch assay at 1, 2, 3 and 4 days of culture. Alkaline phosphatase activity was detected at 4 days of culture. Osteogenic ability was detected by alizarin red staining at 21 days of culture. Cells were continuously passaged three times, and the morphology of osteoblasts was observed under microscope. Cell apoptosis was detected by flow cytometry. RESULTS: In TNF-α group, the shape of osteocytes was irregular, the number of apoptotic cells increased, the migration of osteocytes was slow, the amount of calcium nodules decreased, the activity of alkaline phosphatase decreased, the apoptosis of osteocytes increased and the shape changed during cell passage. The MEM control group, the antimicrobial peptide group and the TNF-α+ antimicrobial peptide group had no change on the morphology, apoptosis, migration, calcium nodules, alkaline phosphatase activity and cell passage of osteoblasts. CONCLUSIONS: Osteoblast apoptosis can be induced by TNF-α, and the osteoblast apoptosis induced by TNF-α can be inhibited by antibacterial peptide.

Key words: Osteoblast, Antibacterial peptide, TNF-α, Apoptosis

CLC Number:

Q28
R782

Xie Limin, Liu Ying, Wang Ru, Liu Dandan, Li Youkun, Zhou Dongsheng, Yang Desheng, Li Xifeng. The inhibitory effect of antibacterial peptides on osteoblast apoptosis induced by TNF-α[J]. China Journal of Oral and Maxillofacial Surgery, 2025, 23(4): 325-330.

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