Expression changes and clinical value of miR-155-5p, miR-138, miR-381-3p in oral squamous cell carcinoma

doi:10.19438/j.cjoms.2025.02.009

Abstract

Abstract: PURPOSE: To investigate the expression and clinical value of microRNA-155-5p (miR-155-5p), miR-138 and miR-381-3p in oral squamous cell carcinoma(OSCC). METHODS: Cancer tissues and adjacent normal tissues of 72 patients with OSCC were collected. Quantitative real-time PCR(RT-PCR) was used to detect the mRNA expressions of miR-155-5p, miR-138 and miR-381-3p, and to analyze the relationship between the expressions of miR-155-5p, miR-138 and miR-381-3p and clinicopathological characteristics and prognosis in OSCC. SPSS 25.0 software package was used for data analysis. RESULTS: Compared with adjacent normal tissues, the expression of miR-155-5p in cancer tissues was significantly increased, and the expression of miR-138 and miR-381-3p was significantly decreased(P< 0.05). High expression of miR-155-5p mRNA and low expression of miR-138 and miR-381-3p mRNA were found in cancer tissues of patients with tumor maximum diameter ≥ 3 cm, tumor invasion depth ≥ 5 mm, clinical stage Ⅲ-Ⅳ, cervical lymph node metastasis and moderate to high differentiation(P< 0.05). After 2 years of follow-up, the survival rate of 72 patients with OSCC was 40.32%(25/72). Compared with the survival patients, the expression of miR-155-5p mRNA in the dead patients was higher, and the expression of miR-138 and miR-381-3p mRNA was lower. Receiver operating characteristic (ROC) analysis showed that the area under the curve(AUC) of miR-155-5p, miR-138 and miR-381-3p combined to predict poor prognosis in patients with OSCC was 0.957, which was significantly higher than that of single mRNA detection (P< 0.05). CONCLUSIONS: The expression of miR-155-5p in OSCC tissues is high, while miR-138 and miR-381-3p expression are low. The expression of the three factors are related to the invasion depth, clinical stage, cervical lymph node metastasis, differentiation degree and prognosis of the tumor. Clinically, combined detection of the three indicators can be strengthened to evaluate the prognosis of patients.

Key words: Oral squamous cell carcinoma, miR-155-5p, miR-138, miR-381-3p, Clinicopathological features, Prognosis

CLC Number:

R739.8

WANG Xin, JIN Cheng, CUI Huan-huan. Expression changes and clinical value of miR-155-5p, miR-138, miR-381-3p in oral squamous cell carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2025, 23(2): 159-164.

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