Effects of Sophora japonica extract on osteogenic differentiation of bone marrow mesenchymal stem cells

doi:10.19438/j.cjoms.2024.01.004

Abstract

Abstract: PURPOSE: To investigate the effect of Sophora japonica extract on osteogenic differentiation of jaw bone marrow mesenchymal stem cells (jBMSCs). METHODS: The effects of 10, 25, 50 and 100 μmol/L Sophora japonica extract solutions on proliferation of jBMSCs were detected by CCK-8. BCIP/NBT alkaline phosphatase and alizarin red staining were used to detect the effect of 10, 25, 50 μmol/L extracts of Sophora on osteogenic differentiation of jBMSCs. Western blot analysis and real-time quantitative PCR were used to detect the effects of Sophora japonica extract solution on protein and mRNA expression levels of osteogenic differentiation-related genes of jBMSCs. GraphPad Prism 9 software package was used for data analysis. RESULTS: The experimental results showed that 100 μmol/L Sophora japonica extract solution had obvious toxic effect on jBMSCs(P<0.0001); while 10, 25 and 50 μmol/L Sophora japonica extract solution did not affect cell proliferation(P>0.05). Compared with the control group, alkaline phosphatase activity, number of calcium nodules, and protein and mRNA expression levels of osteogenic differentiation-related molecules in the 10, 25 and 50 μmol/L groups were gradually increased. CONCLUSIONS: The extract of Sophora japonica on 10, 25, 50 μmol/L can enhance the osteogenic differentiation of jBMSCs.

Key words: Sophora japonica extract, Jaw bone marrow mesenchymal stem cells, Osteogenic differentiation, Cell proliferation

LIN Hui, GAO Yun-fei, LIU Jing, MA Xu-hui. Effects of Sophora japonica extract on osteogenic differentiation of bone marrow mesenchymal stem cells[J]. China Journal of Oral and Maxillofacial Surgery, 2024, 22(1): 23-28.

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