Effect of salidroside on the growth of oral squamous cell carcinoma cells through regulating NRF2/KEAP1 pathway

doi:10.19438/j.cjoms.2024.05.002

Abstract

Abstract: PURPOSE: To investigate the effect and mechanism of salidroside on the growth of oral squamous cell carcinoma cells. METHODS: WSU-HN30 cells were divided into three salidroside treatment groups, low-dose group(40 μg/mL), middle-dose group(60 μg/mL) and high-dose group(100 μg/mL). The proliferation, migration and apoptosis of WSU-HN30 cells were detected by MTT assay, Transwell assay and flow cytometry, and the mRNA and protein expressions of nuclearfactor erythroidderived 2-like 2 (NRF2) and kelch-like ECH associated protein 1 (KEAP1) were detected by real-time quantitative PCR(RT-PCR) and Western blot. SPSS 20.0 software package was used for data analysis. RESULTS: At the same concentration, the proliferation inhibition rate of salidroside after 48 h was significantly higher than that after 24 h (P<0.05). At the same time, with the salidroside concentration increasing, the proliferation inhibition rate was significantly increased(P<0.05). Compared with blank control group, salidroside treatment groups had significant decrease in the number of cell migration, significant increase in the rate of apoptosis, and significant decrease in mRNA and protein expressions of NRF2 and KEAP1; Moreover, the above changes showed a concentration-dependent manner(P<0.05). CONCLUSIONS: Salidroside can significantly inhibit the proliferation and migration of oral squamous cell carcinoma cells, and induce cell apoptosis, and its mechanism may be related to the down-regulation of expression of NRF2/KEAP1 pathway.

Key words: Oral squamous cell carcinoma, Salidroside, NRF2/KEAP1 pathway, Proliferation, Migration, Apoptosis

CLC Number:

R739.8

CHEN Hua, LI Jing, LUO Mei. Effect of salidroside on the growth of oral squamous cell carcinoma cells through regulating NRF2/KEAP1 pathway[J]. China Journal of Oral and Maxillofacial Surgery, 2024, 22(5): 424-429.

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