Effect of Annexin A1 expression on chemoagents of docetaxel, cisplatin, and 5-fluorouracil for oral squamous cell carcinoma

Abstract

Abstract: PURPOSE: To investigate the effect of Annexin A1 expression on chemoagents of docetacel, cisplatin, and 5-fluorouracil (TPF) in oral squamous cell carcinoma. METHODS: Annexin A1 siRNA retroviral vector was constructed and transfected into oral squamous cell carcinoma cell lines to observe the effect of Annexin A1 siRNA on cellular proliferation and cellular cytotoxicity of chemoagents of TPF. Western blot was used to analyze the protein expression when the cells treated with Annexin A1 intervention and chemoagents, especially on PI3K/AKT associated signal pathway as well as cellular apoptosis. SPSS 18.0 software package was used for two-way analysis of variance. RESULTS: Downregulation of Annexin A1 enhanced the ability of cellular proliferation, inhibited the P27kip1 expression through PI3K/AKT signal pathway, increased the sensitivity of cytotoxicity to chemoagents of TPF, and promoted cell apoptosis in oral squamous cell carcinoma cell line. CONCLUSIONS: Decreased Annexin A1 expression promotes cell proliferation, sensitivity to chemoagents of TPF, and cell apoptosis in oral squamous cell carcinoma.

Key words: Oral squamous cell carcinoma, Sensitivity to chemoagents, Cell proliferation, Cell apoptosis

CLC Number:

R739.8

SUN Wen-wen, TANG Xiao, FU Yong, JU Wu-tong, LIU Ying, TAN Yi-ran, ZHU Dong-wang, MA Jie, MA Hai-long, TU Yao-yao, WANG Min, ZHONG Lai-ping, ZHANG Zhi-yuan. Effect of Annexin A1 expression on chemoagents of docetaxel, cisplatin, and 5-fluorouracil for oral squamous cell carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(4): 295-299.

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