China Journal of Oral and Maxillofacial Surgery ›› 2024, Vol. 22 ›› Issue (5): 417-423.doi: 10.19438/j.cjoms.2024.05.001

• Original Articles • Previous Articles     Next Articles

Effects of platelet-derived growth factor D on migration and odontogenic differentiation of human dental pulp stem cells

LIAO Yin-xiu1, ZHANG Mao-lin1, ZOU Duo-hong1,2   

  1. 1. Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011;
    2. Anhui Key Laboratory of Oral Diseases Research, School of Stomatology, Affiliated Stomatology Hospital of Anhui Medical University. Hefei 230032, Anhui Province, China
  • Received:2024-02-07 Revised:2024-04-05 Online:2024-09-20 Published:2024-09-29

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Abstract: PURPOSE: To investigate the effect of human recombinant platelet-derived growth factor D(PDGFD) on migration and odontogenic differentiation of human dental pulp stem cells(hDPSCs). METHODS: Primary hDPSCs were isolated by enzymolysis and cultured. The expression of molecular markers on the surface of cultured mesenchymal stem cells was identified by flow cytometry. Three lines of hDPSCs were induced and identified by corresponding staining to characterize their potential for multidirectional differentiation. The effect of PDGFD on the migration ability of hDPSCs was investigated by cell scratching test. RT-PCR and Western blotting were used to detect the effects of PDGFD on the expression of odontoblast-related mRNA and proteins in hDPSCs. Alkaline phosphatase(ALP) and alizarin red staining(ARS) were used to detect the effect of PDGFD on the mineralization of hDPSCs. SPSS 26.0 software package was used for data analysis. RESULTS: Morphological analysis, flow cytometry identification and three-line differentiation showed that the isolated cells were consistent with the characteristics of hDPSCs and had multidirectional differentiation potentials. The results of cell scratching test showed that only 50 ng/mL PDGFD at 12 h had an effect on the migration ability of hDPSCs, but both 10 and 50 ng/mL PDGFD at 24 h had an effect on the migration ability of hDPSCs. PCR results showed that PDGFD of 10 and 50 ng/mL could promote the odontogenic differentiation of hDPSCs, and PDGFD of 50 ng/mL had a more significant effect on the odontogenic differentiation of hDPSCs. The results of Western blotting, ALP and ARS were the same as those of PCR. CONCLUSIONS: hDPSCs with typical mesenchymal stem cell phenotype and multidirectional differentiation potential were successfully isolated and cultured. PDGFD at the concentration of 10 ng/mL and 50 ng/mL can promote the migration and odontogenic differentiation of human dental pulp stem cells, and the promotion effect of PDGFD at the concentration of 50 ng/mL is more significant.

Key words: Platelet-derived growth factors D, PDGFD, Human dental pulp stem cells, hDPSCs, Odontogenic differentiation, Cell migration

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