Expression of Smad7 protein in oral squamous cell carcinoma and the impact on cell proliferation and migration of oral squamous cell carcinoma

doi:10.19438/j.cjoms.2017.03.006

Abstract

Abstract: PURPOSE: To investigate the expression of Smad7 in oral squamous cell carcinoma (OSCC) and determine if Smad7 was associated with tumor cell proliferation and migration. METHODS: Thirty-one primary OSCC patients were enrolled in this study. Smad7 expression in tissue specimens were investigated by using immunohistochemistry staining, CCK-8, cell scratch assay, western blotting and qRT- PCR were performed after Smad7 siRNA interference in HN4 cells. Paired t test was used for analysis with SPSS18.0 software package. RESULTS: Nuclear and cytoplasmic expression of Smad7 was increased in primary OSCC tissue samples. Expression of Smad7 was associated with cervical lymph node metastasis. Smad7 knockdown significantly suppressed cell proliferation but promoted tumor cell migration in HN4 cells. Smad7 knockdown promoted N-cadherin and Vimentin expression. CONCLUSIONS: Smad7 expression in OSCC was associated with cervical lymph nodal metastasis and Smad7 might play an essential role in the proliferation and migration of OSCC cells.

Key words: Smad7 protein, Oral squamous cell carcinoma, Western blot, RNA interference

CLC Number:

R783.3

ZHENG Chen, SHI Chao-ji, ZHENG Bo, JIN Xiao-ming, JIANG Yin-hua.. Expression of Smad7 protein in oral squamous cell carcinoma and the impact on cell proliferation and migration of oral squamous cell carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(3): 220-226.

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