Expression and functional role of miR-214 in oral squamous cell carcinoma

Abstract

Abstract: PURPOSE: To evaluate the expression of miR-214 and investigate its functional role in oral squamous cell carcinoma (OSCC). METHODS: RT-PCR was performed to detect miR-214 expression levels in 31 OSCC tissues and 7 OSCC cell lines. The correlation of miR-214 expression with the clinicopathologic features of the patients was analyzed with Chi-square test. Then, OSCC cells were transfected with miR-214 mimics and inhibitors. Wound healing assay and Transwell assay were performed to determine cell migratory and invasive abilities. Flow cytometry was used to assess cell cycle and apoptosis. SPSS19.0 software package was used for statistical analysis. RESULTS: In 31 OSCC patients, the expression of miR-214 positively correlated with lymph node metastasis and clinical stage (P<0.05). In vitro, the invasion and migration abilities were substantially enhanced in UM1 cells (P<0.05) transfected with miR-214 mimics and decreased (P<0.05) in SCC9 cells transfected with miR-214 inhibitors. Cell cycle was arrested in G1 stage but the apoptosis was not significantly increased in UM1 cells treated with miR-214 mimics. However, there were no obvious changes on cell cycle and apoptosis in SCC9 cells treated with miR-214 inhibitors. CONCLUSIONS: miR-214 is correlated with cervical lymph node metastasis and clinical stage in OSCC. It may act as an oncomiRNA to promote invasion and migration in OSCC.

Key words: miR-214, Oral squamous cell carcinoma, Invasion, Migration

CLC Number:

R739.8

WU Yue, WANG Cheng, XIE Nan, ZHUANG Ze-hang, LIU Xi-qiang, HOU Jin-song, HUANG Hong-zhang. Expression and functional role of miR-214 in oral squamous cell carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2016, 14(2): 97-105.

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