Cancer-associated fibroblasts induce epithelial-to-mesenchymal transition in tongue squamous cell carcinoma

Abstract

Abstract: PURPOSE: To examine the role of cancer-associated fibroblasts (CAFs) in promoting invasion of tongue squamous cell carcinoma (TSCC) and the possible mechanism. METHODS: A series of matched pairs of CAFs and normal fibroblasts (NFs) were primarily cultured in collagenase digestion. Identified markers of alpha smooth muscle actin (α-SMA), stromal cell-derived factor-1(SDF1), vimentin and cytokeratin (CK) were observed. Transwell assay, Western blot, reverse transcription-PCR and immunofluorescence staining were carried out to assess the role of CAFs in promoting migration, invasion and inducing epithelial to mesenchymal transition (EMT) of SCC9, compared with NFs. RESULTS: Both primarily cultured CAFs and NFs expressed vimentin, but not CK. CAFs showed significantly higher α-SMA expression and SDF1 secretion, as well as mRNA level of α-SMA and SDF1. Co-culture with CAFs induced up-regulation of EMT markers (fibronectin and vimentin) and down-regulation of E-cadherin of SCC9, as well as enhanced invasion. CONCLUSION: Collagenase digestion is a reliable way to primary culture of CAFs. CAFs may induce EMT markers and functional changes in SCC9. Supported by National Natural Science Foundation of China (81172563).

Key words: Cancer-associated fibroblast, Tumor microenvironment, Tongue squamous cell carcinoma, Epithelial-mesenchymal transition

CLC Number:

R739.8

ZHOU Bin, CHEN Wei-liang, WANG You-yuan, LIN Zhao-yu, ZHANG Da-ming, FAN Song. Cancer-associated fibroblasts induce epithelial-to-mesenchymal transition in tongue squamous cell carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2014, 12(3): 198-204.

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