Effect of long noncoding RNA FOXD2-AS1 on migration and invasion of tongue squamous cell carcinoma: an in vitro study

doi:10.19438/j.cjoms.2020.06.005

Abstract

Abstract: PURPOSE: To investigate the effects of long noncoding RNA FOXD2-AS1 on migration and invasion of tongue squamous cell carcinoma (TSCC). METHODS: RT-qPCR was carried out to validate the expression of lncRNA FOXD2-AS1 in 35 paired clinical TSCC tissues, the relationship between FOXD2-AS1 expression and clinicopathological characteristics was analyzed with the 8th version of UICC TNM staging criteria. Receiver operating characteristic curve (ROC) was made according to RT-qPCR data. Transwell migration and invasion, colony formation assays were performed to detect migration, invasion and proliferative ability of TSCC cells after FOXD2-AS1 being downregulated in SCC-9 and CAL-27 cell lines. Western blot was conducted to detect the influence that FOXD2-AS1 downregulation might have on downstream molecules. SPSS 23.0 software package was used to analyze the data. RESULTS: Compared to para-carcinoma tongue tissues, lncRNA FOXD2-AS1 was highly expressed in TSCC tissues and its high expression was associated with cervical lymph node metastasis and poor TNM stages. In ROC, area under curve (AUC) was 0.8122,95% CI was 0.7141-0.9103,sensitivity and specificity were 0.6 and 0.8857, respectively, and the value of cutoff was 4.122. After FOXD2-AS1 being downregulated in SCC-9 and CAL-27 cell lines,Transwell migration and invasion assays revealed that migration and invasion of TSCC cells were inhibited, but the proliferative ability remained unchanged. Western blot indicated that the expression of β-catenin in Wnt/β-catenin signal pathway downregulated after lncRNA FOXD2-AS1 knockdown. CONCLUSIONS: lncRNA FOXD2-AS1 is highly expressed in TSCC, which promotes migration and invasion of TSCC and is associated with cervical lymph node metastasis and poor TNM stages, probably through modulating Wnt/β-catenin signal pathway.

Key words: Tongue squamous cell carcinoma, LncRNA FOXD2-AS1, Migration, Invasion, Wnt/β-catenin signal pathway

CLC Number:

R739.8

ZHOU Guang-ming, LIANG Yan-can, HUANG Zi-xian, ZHANG Bin. Effect of long noncoding RNA FOXD2-AS1 on migration and invasion of tongue squamous cell carcinoma: an in vitro study[J]. China Journal of Oral and Maxillofacial Surgery, 2020, 18(6): 495-500.

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