Effects of risedronates on Bcl-2 and BAX expression in osteoporotic rat mandibular alveolar bone

doi:10.19438/j.cjoms.2018.05.004

Abstract

Abstract: PURPOSE: This study was to investigate the impact of risedronates on mandibular alveolar bone microstructure, anti-apoptotic factor Bcl-2 and apoptotic factor BAX expression and osteocyte apoptosis in an ovariectomized rat model. METHODS: Thirty 6-month-old female SD rats were divided into 3 groups (ten in each group): Sham group, OVX group (bilateral ovariectomy) and RIS group (bilateral ovariectomy, followed by administration of risedronate). Three months later, the mandibles of all animals were harvested and assessed by micro-CT and histological analysis. Osteocyte apoptosis was observed by TUNEL staining and the expression of Bcl-2 and BAX was evaluated by immunohistochemical staining. The data were analyzed using SPSS 13.0 software package. RESULTS: Ovariectomy caused significant alveolar bone loss and greater osteocyte apoptosis in OVX group than in sham group. The systemic risedronate-treated group exhibited a significant increase in BV/TV and decrease in trabecular separation and osteocyte apoptosis, together with an increase in Bcl-2 expression and the ratio of Bcl-2/BAX, when compared with those of the OVX group (P<0.05). CONCLUSIONS: Systemic treatment of risedronates can increase the expression of Bcl-2 and the ratio of Bcl-2/BAX in mandibular alveolar bone. It partially reverses alveolar bone loss and suppresses osteocyte apoptosis in ovariectomized rats.

Key words: Ovariectomy, Mandibular alveolar bone, Bisphosphonates, Osteoporosis, Osteocyte apoptosis

CLC Number:

R782.1

WEI Li, ZHOU Qi, JIANG Li-ting, LI Ning, GAO Yi-ming. Effects of risedronates on Bcl-2 and BAX expression in osteoporotic rat mandibular alveolar bone[J]. China Journal of Oral and Maxillofacial Surgery, 2018, 16(5): 402-408.

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