Effect of silencing circular RNA hsa_circ_0006677 on migration and invasion of oral squamous cell carcinoma

doi:10.19438/j.cjoms.2019.04.001

Abstract

Abstract: PURPOSE: To investigate the role of aberrantly expressed hsa_circ_0006677 in oral squamous cell carcinoma (OSCC) and its effect on OSCC migration and invasion. Methods: Quantitative PCR (qRT-PCR) was used to validate hsa_circ_0006677 in 40 pairs of matched OSCC tissue samples. The correlation between hsa_circ_0006677 expression and clinical information and receiver operating characteristic (ROC) curve was analysed. qRT-PCR assay was used to detect the expression of hsa_circ_0006677 in OSCC cell lines (SCC-9, SCC-15, SCC-25, CAL-27) and human oral keratinocytes (HOK). Scratch test and Transwell assay were used to evaluate the effect of silencing hsa_circ_0006677 on invasion and migration of OSCC cells SCC-15. The data were statistically analysed with Graphpad 5.0 software. Results: Hsa_circ_0006677 was significantly down-regulated in OSCC tissues compared with matched normal tissues (P<0.001), and the relative expression of hsa_circ_0006677 was correlated with cervical lymph node metastasis (P= 0.0405). The area under ROC curve (AUC) was 0.8221. The cutoff value was 10.74, the sensitivity and specificity were 0.8095 and 0.7619, respectively. The expression of hsa_circ_0006677 in SCC-15 and CAL-27 was significantly decreased. After transfecting si-hsa_circ_0006677 into SCC-15 cells, the ability of invasion and metastasis was significantly improved. Conclusions: The circular RNA hsa_circ_0006677 is significantly down-regulated in OSCC. Silencing hsa_circ_0006677 can significantly promote metastasis and invasion of SCC-15 cells.

Key words: Circular RNA, Hsa_circ_0006677, Oral squamous cell carcinoma, Metastasis, Invasion

CLC Number:

R739.8

LI Xiang, SU Wen, ZHANG Han-yu, ZHANG Bi-ru, YANG Hong-yu. Effect of silencing circular RNA hsa_circ_0006677 on migration and invasion of oral squamous cell carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2019, 17(4): 289-294.

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