Research on wound healing and variation of hypertrophic scar in rabbit after cleft lip surgery

doi:10.19438/j.cjoms.2017.06.003

Abstract

Abstract: PURPOSE: To observe the hyperplasia trend of hypertrophic scar after cleft lip surgery in rabbits, and to explore the exact time of the largest degree of hypertrophic scar after cleft lip repair, which provided a theoretical basis for the study of the most effective time of gene therapy for hypertrophic scars. METHODS: Forty New Zealand white rabbits from the same offspring were used to make wound healing model in cleft lip Millard's procedure. The volumes were measured respectively and granulation tissues were observed visually at 2nd week, 3rd week, 4th week and 5th week after operation. The rabbits were sacrificed and scar tissues were harvested in the indicated time. Immunohistochemical paraffin section (IHC-P), myofibroblast (MFB) cell count and Western blot were performed to detect the expression level of α-smooth muscle actin (α-SMA) in the scar tissue. SPSS 18.0 software package was used for statistical analysis. RESULTS: The results showed that the scar contraction was significant at 3rd and 4th week, comparing to 2nd and 5th week after surgery. The degree of scar hyperplasia after surgery was approximately in normal distribution. CONCLUSIONS: MFBs were important cells in scar formation and α-SMA was an important marker of MFBs. The study provides an objective basis for determination of scar severity by measuring the protein expression of α-SMA.

Key words: Rabbit, Cleft lip, Hypertrophic scar, Myofibroblast, α-smooth muscle actin

CLC Number:

R782.2

LIU Feng, ZHANG Dai-zun, XIAO Wen-lin, XUE Ling-fa, XU Yao-xiang. Research on wound healing and variation of hypertrophic scar in rabbit after cleft lip surgery[J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(6): 493-497.

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