Evaluation of neurogenic bone regeneration of thermosensitive bFGF/Dex liposome hydrogel composite scaffold

doi:10.19438/j.cjoms.2024.04.002

Abstract

Abstract: PURPOSE: Liposomes were used to simultaneously encapsulate basic fibroblast growth factor (bFGF) that can promote nerve differentiation and osteogenic small molecule (Dex). Then, a temperature sensitive and injectable bFGF/Dex liposome-hydrogel composite sustained-release scaffold was constructed by mixing it with sodium alginate solution. METHODS: bFGF/Dex liposomes were prepared by film dispersion method, mixed with sodium alginate solution activated by RGD polypeptide, and thermosensitive bFGF/Dex liposome hydrogel composite scaffolds were synthesized by free radical polymerization. The particle size, polydispersity, and Zeta potential of liposomes were detected by Zeta particle size analyzer. Microstructure of the scaffold was observed under scanning electron microscopy, and mechanical strength of the scaffold was measured using a universal material testing machine. Real time fluorescence quantitative PCR (RT-PCR) and immunofluorescence staining were used to detect the expression of bFGF promoting neural differentiation related genes and proteins in hBMSCs. Rabbit skull defect models were constructed and the effect of the composite scaffold on promoting neurogenic bone regeneration in vivo was evaluated through micro-CT and immunohistochemical staining. SPSS 26.0 software package was used for statistical analysis. RESULTS: Thermosensitive bFGF/Dex liposome hydrogel composite scaffold was successfully constructed. It was in a solution state and had fluidity at normal temperature(25℃), and quickly changed to a gel state when higher than the critical temperature(32 ℃). The surface of scaffold was loose and porous, which could bear certain stress. The expression of genes and proteins related to neurogenesis suggested that bFGF had a strong promoting effect on neurogenesis in hBMSCs. The results of animal experiments showed that the composite had a good neurogenic bone regeneration effect. CONCLUSIONS: The intelligent bFGF /Dex liposome hydrogel composite scaffold with temperature sensitivity and outstanding biological function provides a new material for repair of jaw defects.

Key words: Jaw bone defect, Bone graft materials, Neurogenic bone regeneration, Temperature sensitivity composite scaffold

CLC Number:

R782

CHU Chen, ZONG Qian, XUN Xing-xiang, ZHAO Qian, YUAN Rong-tao, XU Xiao. Evaluation of neurogenic bone regeneration of thermosensitive bFGF/Dex liposome hydrogel composite scaffold[J]. China Journal of Oral and Maxillofacial Surgery, 2024, 22(4): 322-328.

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