Bone remolding after adjacent tooth moving into alveolar cleft site repaired by tissue-engineered bone graft

Abstract

Abstract: PURPOSE: To study the biological effects of orthodontic tooth movement into surgical site on bone remodeling after bone graft. METHODS: Twelve SD rats were used as experimental animals. Critical size defects (CSD) were established on maxillary alveolar bone in SPF SD rats (8-week-old, 200±20g). 3D printed polycaprolactone scaffolds and rat bone marrow mesenchymal stem cells were co-cultured in vitro and then were used to be grafted into CSDs as substitution of autogenous bone. Eight weeks after surgery, 3 rats were sacrificed to take samples. H-E staining was used to evaluate the osteogenesis. Meanwhile, orthodontic devices were used to move adjacent teeth into surgical area in one side, the other side was used as control. Three SD rats were sacrificed respectively 1, 3 and 7 days after treatment. Real-time PCR was used to detect the expression of OPN, Trap, Runx2, Rank1, OCN and VEGF between the experimental group and control group. SPSS 19.0 software package was used for statistical analysis. RESULTS: H-E staining revealed abundant bone regeneration and osseous tissue was mature. RT-PCR showed that OCN expressed highest in late stage but had no significant difference between early stage and middle stage; expression of Runx2 rose from early stage to middle stage and declined to late stage; VEGF expression progressively decreased from early stage to late stage; expressions of each gene in the experimental group were significantly higher than the control group (P<0.05). CONCLUSIONS: Tissue-engineered bone employing 3D printed scaffold can successfully substitute for autogenous bone to regenerate new bone. Using orthodontic method to move adjacent tooth into surgical area can promote bone regeneration, induce osteoblast and osteoclast to remodel bone biological structure.

Key words: Alveolar cleft, 3D printing, Tissue engineering, RT-PCR, Bone remodeling

CLC Number:

R782.1

JIN Can, CHEN Zhen-qi, WU Jun. Bone remolding after adjacent tooth moving into alveolar cleft site repaired by tissue-engineered bone graft[J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(4): 310-313.

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