Research on the co-culture model of salivary adenoid cystic carcinoma cells and rat schwann cells

Abstract

Abstract: PURPOSE : To establish a co-culture system of salivary adenoid cystic carcinoma (SACC) cells with rat schwann cells for further investigating the changes of brain-derived neurotrophic factor (BDNF), tropomysin-related kinase B (TrkB) and E-cadherin after cells interaction. METHODS : Transwell system was used to establish co-culture model of SACC cells with rat schwann cells. After co-culture for 96 hours, the morphology of tumor cells were investigated, the change of BDNF in co-culture medium of each group was tested by enzyme-linked immunosorbent assay (ELISA) and the expression of BDNF, TrkB and E-cadherin in tumor cells were detected by RT-PCR and Western blot. The date was analyzed using SPSS 17.0 software package. RESULTS : Compared with the single culture group, the morphology of SACC cells co-cultured with schwann cells changed to spindle-shape and polygon-shape; the expression of E-cadherin in co-cultured SACC cells was down-regulated while the expression of TrkB and phospho-TrkB was up-regulated, but the expression of BDNF had no obvious change; more expression of BDNF was detected in the co-culture medium secreted by schwann cells. The mucoepidermoid carcinoma (MEC) cells in the control group, co-cultured with schwann cells, had no similar changes. CONCLUSIONS : BDNF/TrkB signal pathway plays an important role in the process of perineural invasion of SACC, but the concrete molecular mechanism needs further experimental investigation.

Key words: Salivary adenoid cystic carcinoma, Schwann cells, Co-culture, BDNF, TrkB, E-cadherin, Perineural invasion

CLC Number:

R739.8

SHAN Chun, YANG Xin-jie, WU Bao-lei, WANG Xin-ge, LEI De-lin.. Research on the co-culture model of salivary adenoid cystic carcinoma cells and rat schwann cells[J]. China Journal of Oral and Maxillofacial Surgery, 2015, 13(6): 481-485.

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