Effect of simvastatin combined with simiR-21 on migration and invasion of human salivary adenoid cystic carcinoma

doi:10.19438/j.cjoms.2018.02.007

Abstract

Abstract: PURPOSE: To investigate the effect of simvastatin combined with miR-21 on migration and invasion of human salivary adenoid cystic carcinoma cell line (SACC-LM) and its underlying mechanism. METHODS: SACC-LM cells were divided into 4 groups randomly. In NC group, unrelated nucleotides sequence was transfected and a blank reagent was added; in SIM group, unrelated nucleotides sequence was transfected and simvastatin was added; in simiR-21 group, miR-21 inhibitor was transfected and a blank reagent that did not contain simvastatin was added; in simiR-21+SIM group, miR-21 inhibitor was transfected and simvastatin was added. qRT-PCR was used to detect the expression of miR-21. CCK-8 method was used to measure the IC₅₀ of SACC-LM while simvastatin (10,20,30,40,50,60 μmol/L) was added for 48 h. Transwell chamber method was used to observe the migration (without matrigel) and invasion (adding matrigel) ability. Western blot was used to detect the expression of EMT relative proteins (E-Cadherin, Snail1). Student's t test or one-way ANOVA were performed to analyze the data using SPSS 22.0 software package. RESULTS: The expression of miR-21 was remarkably down-regulated in the groups with transfected inhibitor(P<0.01). Simvastatin had no effect on the expression of miR-21(P>0.05). Simvastatin could inhibit the proliferation of SACC-LM cells in a dose-dependent manner. miR-21 was effective in reducing the resistance of SACC-LM to simvastatin. The abilities of migration and invasion on SACC-LM cells in simiR-21+SIM group were significantly inhibited compared with other groups (P<0.01). The expression of E-cadherin in the combined group was significantly higher than other groups (P<0.01) with decreased Snail1 expression(P<0.001). CONCLUSIONS: MiR-21 inhibitor transfection can effectively reduce the resistance of SACC-LM to SIM. In simiR-21+SIM group, the abilities of migration and invasion on SACC-LM cells were more greatly inhibited than other groups, indicating the mechanism may be related to down-regulation of Snail1 expression and up-regulation of E-Cadherin expression.

Key words: Simvastatin, MicroRNA-21, Salivary adenoid cystic carcinoma, E-cadherin, Snail1

CLC Number:

R739.8

WANG Chao, LI Ting, YAN Fei, CAI Wen-yan, JIANG Xing-yu, SUN Jin-hu. Effect of simvastatin combined with simiR-21 on migration and invasion of human salivary adenoid cystic carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2018, 16(2): 132-137.

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