Construction and in vitro evaluation of an injectable multifunctional liposome-hydrogel composite for jaw defect repair

doi:10.19438/j.cjoms.2022.06.003

Abstract

Abstract: PURPOSE: Liposome was used as a sustained release carrier for pro-vascularization small molecule desferrioxamine (DFO) and osteogenesis-inducing small molecule dexamethasone (Dex), while sodium alginate hydrogel was used as a sustained release carrier for vancomycin (Van) to construct an injectable liposome-hydrogel complex with simultaneous antibacterial, pro-angiogenic and osteogenic effects, and to investigate the ability of the complex to promote bone tissue regeneration and repair. METHODS: DFO/Dex liposomes were prepared by thin film dispersion method, and mixed with Van sodium alginate solution to form Van/DFO/Dex liposome-hydrogel complex under the action of calcium ions. The particle size, polydispersity index(PDI) and Zeta potential of the liposomes were measured by Zeta particle size analyzer. The sustained release curves of Van, DFO and Dex in the complex were determined by UV-vis spectrophotometer. Wst-8 quantitative assay was used to evaluate the antibacterial activity of the complex in vitro. CCK-8 and cell live/dead fluorescence staining were used to evaluate the cytocompatibility of the complex. Real-time fluorescence quantitative PCR (RT-PCR) was used to detect the angiogenesis promoting effect of the complex. ALP activity quantification and staining were used to evaluate the effect of Van/DFO/Dex liposome-hydrogel complex on promoting bone differentiation in vitro. OriginPro software was used for analysis of the data. RESULTS: An injectable Van/DFO/Dex liposome-hydrogel complex was successfully constructed. The particle size of the liposome was (121.6±6.35)nm, PDI was 0.2-0.3. The encapsulation rate of DFO was about 57% and that of Dex was about 23%. Sustained release curve showed that Van DFO and Dex were released continuously in stages. CCK-8 and cell live/dead fluorescence staining showed that Van/DFO/Dex liposome-hydrogel complex had good cytocompatibility. Quantitative detection of WST-8 showed that the complex had good antibacterial activity in vitro. The expression of genes related to angiogenesis suggests that the complex had the ability to promote angiogenesis. ALP activity quantification and staining showed that Van/DFO/Dex liposome-hydrogel complex could enhance osteogenic differentiation activity. CONCLUSIONS: Van/DFO/Dex liposome-hydrogel complex is a multifunctional bone regeneration material with osteogenesis, angiogenesis promotion and anti-infection, and its injectable properties are beneficial to the clinical application of irregular jaw defects.

Key words: Jaw defect, Bone substitute, Angiogenesis, Osteogenesis, Bacteriostasis

CLC Number:

R782.4

XUN Xing-xiang, SUN Wei, WANG He-jing, CHU Chen, ZHAO Qian, XU Xiao, YUAN Rong-tao. Construction and in vitro evaluation of an injectable multifunctional liposome-hydrogel composite for jaw defect repair[J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(6): 534-540.

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