Identification and genome-wide analysis of C. sputigena isolated from squamous cell carcinoma of the tongue

doi:10.19438/j.cjoms.2024.06.002

Abstract

Abstract: PURPOSE: To isolate and identify Capnocytophaga sputigena(C. sputigena) from tongue squamous cell carcinoma(TSCC), then analyze the drug resistance and virulence genes at genome-wide level. METHODS: The bacterial strains n105 and n106 were isolated and cultured from the cancer tissue mucosa of TSCC patients. Bacterial species were determined through colony morphology, Gram staining, 16S rRNA sequence, and construction of evolutionary tree. Then the drug sensitivity of bacterial strains to 4 antibiotics(clarithromycin, metronidazole, penicillin and levofloxacin) was detected by disk diffusion method. Whole genome sequencing and functional annotation of virulence and resistance genes were performed on strain n106 by the third-generation sequencing technology. RESULTS: Strains n105 and n106 were identified as C. sputigena through 16S rRNA sequence BLAST alignment and construction of Neighbor Joining evolutionary trees. The results of drug sensitivity test showed that C. sputigena strains n105 and n106 were sensitive to clarithromycin, while resistant to metronidazole, penicillin, and levofloxacin. Whole genome sequencing results showed that the genome length of C. sputigena strain n106 was 2.86 Mb, with 38.36% of guanine cytosine(GC) content, encoding 2 609 genes. Six virulence genes (tufA, app, clpV, cpsA, acpXL and fHbp) and two drug resistance genes [blaCSP-1and erm(F)] were predicted. CONCLUSIONS: C. sputigena isolated from TSCC is resistant to metronidazole, penicillin, and levofloxacin, carrying multiple virulence and resistance genes, which possessing potential pathogenic characteristics. This study provides new strategies for effective prevention, control, and treatment of TSCC from a microbial perspective.

Key words: Capnocytophaga sputigena, Tongue squamous cell carcinoma, Genomics, Drug resistance, Virulence genes

CLC Number:

R739.8

GUAN Zhi-hui, LI Ru, YU Xiao-yun, WANG Li-li, YU Miao-miao, GONG Lu-yang, YUAN Rong-tao, ZHOU Jian-hua. Identification and genome-wide analysis of C. sputigena isolated from squamous cell carcinoma of the tongue[J]. China Journal of Oral and Maxillofacial Surgery, 2024, 22(6): 529-535.

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