Generation of Fgf9 conditional knockout mice in cartilage

doi:10.19438/j.cjoms.2019.05.005

Abstract

Abstract: PURPOSE: To establish a conditional knockout mouse model of fibroblast growth factor 9 (FGF9) in cartilage with Cre-LoxP system. METHODS: The recombinant vector of exon 2 of mouse Fgf9 was constructed and transfected into ES cells by electroporation. The positive ES cells were screened by G418 and Ganciclovoir and identified by PCR. ES cells were microinjected into the blastocysts of C57BL/6J mice and then transferred into the uterus of pseudopregnant mice to obtain chimeric mice. Flox region in chondrocytes of flox homozygous and Cre-positive fetuse was knocked out by hybridizing the Neo-free mice with Col II-Cre mice and induced with tamoxifen. RESULTS: Fgf9 Flox heterozygous mice reproduced steadily without obvious abnormality. After mating with Col II-Cre transgenic mice, the Flox mice successfully produced a conditional knockout mouse model of Fgf9 in cartilage tissue. CONCLUSIONS: Conditional knockout mice model of FGF9 was successfully established with Cre-LoxP system, which lays a foundation for further study of the function of FGF9 in osteochondral development and bone and joint homeostasis.

Key words: FGF9, Conditional knockout, Col II-Cre transgenic mice

CLC Number:

R783.5

WENG Meng-jia, ZHANG Xiao-yue, CHEN Zhen-qi. Generation of Fgf9 conditional knockout mice in cartilage[J]. China Journal of Oral and Maxillofacial Surgery, 2019, 17(5): 412-417.

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