Effect of β-NGF on bone formation and bone remodeling during healing of bone defect

doi:10.19438/j.cjoms.2017.03.001

Abstract

Abstract: PURPOSE: Standard rat skull critical bone defects were made and locally continuous infusion of β-NGF animal model was conducted. The effect of β-NGF on bone formation and bone remodeling was evaluated. METHODS: Two 5 mm diameter bone defects were created on the parietal bone of SD rats, the right side was given 10 μg β-NGF PBS solution(experimental group) and the left side was only given PBS solution(control group) via osmotic pump for 7 days. New bone formation was evaluated using H-E staining and Gomori modified trichrome staining. New bone formation in the bone defect of AOI was measured with Image-pro Plus 6.0 software and the data were analyzed using SPSS 19.0 software package. RESULTS: H-E staining showed the new bone formation on the both sides. The results of Gomori modified trichrome staining showed significantly increased new bone formation after application of β-NGF at 21 d and 28 d. The quality of mature bone was significantly higher in the experiment group compared with the control group at 28 d(P<0.05). CONCLUSIONS: Local application of β-NGF during bone healing can promote new bone formation and bone remodeling.

Key words: β-NGF, Bone defects, Bone formation, Bone remodeling

CLC Number:

R782

LU Meng, ZHUO Li-li, MAO Chuan-qing, WANG Jin, WANG Cheng-yong, CHEN Wei-hui.. Effect of β-NGF on bone formation and bone remodeling during healing of bone defect[J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(3): 193-197.

References

[1] Levi-montalcini R, Hamburger V. Selective growth stimulating effects of mouse sarcoma on the sensory and sympathetic nervous system of the chick embryo[J]. J Exp Zool, 1951, 116(2): 321-361.
[2] Hiriart M, Vidaltamayo R, Sánchez-Soto MC. Nerve and fibroblast growth factors as modulators of pancreatic beta cell plasticity and insulin secretion[J]. Isr Med Assoc J, 2001, 3(2): 114-116.
[3] Grills BL, Schuijers JA, Ward AR. Topical application of nerve growth factor improves fracture healing in rats[J]. J Orthop Res, 1997, 15(2): 235-242.
[4] Eppley BL, Snyders RV, Winkelmann TM, et al. Efficacy of nerve growth factor in regeneration of the mandibular nerve: a preliminary report[J]. J Oral Maxillofac Surg, 1991, 49(1): 61-68.
[5] Eppley BL, Snyders RV, Windelmann T, et al. Effects of nerve growth factor on craniofacial onlay bone graft survival: preliminary findings [J]. J Craniofac Surg, 1992, 2(4): 174-180.
[6] Meyer M H, Etienne W, Meyer RA Jr. Altered mRNA expression of genes related to nerve cell activity in the fracture callus of older rats: A randomized, controlled, microarray study [J]. BMC Musculoskelet Disord, 2004, 5: 24.
[7] 中华人民共和国科技部.关于善待实验动物的指导性意见[DB/OL].(2006-09-30)[2016-07-25].http://www.most.gov.cn/fggw/zfwj/zfwj2006/zf06yw/zf06qt/200612/t20061226_39235.htm.
[8] 陈伟辉, 卓丽莉, 茅传青, 等. 大鼠颅骨骨缺损局部持续灌注β神经生长因子给药模型的建立[J]. 福建医科大学学报, 2014, 48(5): 303-307.
[9] 茅传青, 卓丽莉, 王锦, 等. 外源性β-NGF调节骨改建过程中的可能作用机制 [J]. 中国实验动物学报, 2016, 24(1): 59-64.
[10] Lazarovici P, Gazit A, Staniszewska I, et al. Nerve growth factor (NGF) promotes angiogenesis in the quail chorioallantoic membrane [J]. Endothelium, 2006, 13(1): 51-59.
[11] Emanueli C, Salls MB, Pinna A, et al. Nerve growth factor promotes angiogenesis and arteriogenesis in ischemic hindlimbs[J]. Circulation, 2002, 106(17): 2257-2262.
[12] Steinle JJ, Granger HJ. Nerve growth factor regulates human choroidal, but not retinal, endothelial cell migration and proliferation [J]. Auton Neurosci, 2003, 108(1-2): 57-62.
[13] 于一凡, 孙晋民. 神经生长因子与血管生成的相关性研究进展 [J]. 中国现代医药杂志, 2009, 11(8): 138-140.
[14] Ransj?觟 M, Lie A, Mukohyama H, et al. Microisolated mouse osteoclasts express VIP-1 and PACAP receptors [J]. Biochem Biophys Res Commun, 2000, 274(2): 400-404.
[15] Anderson LE, Seybold VS. Calcitonin gene-related peptide regulates gene transcription in primary afferent neurons [J]. J Neurochem, 2004, 91(6): 1417-1429.
[16] Dobnig H, Sipos A, Jiang Y, et al. Early changes in biochemical markers of bone formation correlate with improvements in bone structure during teriparatide therapy [J]. J Clin Endocrinol Metab, 2005, 90(7): 3970-3977.
[17] Jehan F, Naveilhan P, Neveu I, et al. Regulation of NGF, BDNF and LNGFR gene expression in ROS 17/2.8 cells [J]. Mol Cell Endocrinol, 1996, 116(2): 149-156.
[18] Obrant KJ, Ivaska KK, Gerdhem P, et al. Biochemical markers of bone turnover are influenced by recently sustained fracture [J]. Bone, 2005, 36(5): 786-792.
[19] Lyritis G, Boscainos PJ. Calcitonin effects on cartilage and fracture healing [J]. J Musculoskelet Neuronal Interact, 2001, 2(2): 137-142.
[20] Sun W, Sun C, Lin H, et al. The effect of collagen-binding NGF-beta on the promotion of sciatic nerve regeneration in a rat sciatic nerve crush injury model [J]. Biomaterials, 2009, 30(27): 4649-4656.