Expression of HIF-1α and Notch-1 induced by hypoxia after ischemia reperfusion injury in tissue flap

doi:10.19438/j.cjoms.2018.03.004

Abstract

Abstract: PURPOSE: To observe the expression of HIF-1α-Notch signaling pathway after ischemic-reperfusion injury. METHODS: Twenty-four Wistar Rats were randomly divided into blank group(4 mice), sham group(4 mice) and ischemic-reperfusion （I/R） group (16 mice). The rats of both sham group and IR group were operated to make a 4cm×8cm flap on the left abdomina, then the flaps of IR groups suffered from 6 hours of postoperative ischemia. Four rats were executed each time immediately and the 1st, the 3rd , the 7th day after operation, and the tissue samples in I/R groups were collected. The rats in sham group and blank group were executed at the 7th day and tissue samples were collected. mRNA expression levels of HIF-1α, Notch-1 were detected by RT-PCR and protein expression levels were detected by Western blot in I/R group, sham group and blank group. The data were analyzed using SPSS 19.0 software package. RESULTS: Histological damage occurred in I/R group at the 7th day, which was more significantly grave than the sham group and blank group. The expression of HIF-1α, Notch-1 protein in IR group at each time point was significantly higher compared to sham group (P<0.05). The expression of HIF-1α, Notch-1 mRNA in IR group at each time point was significantly higher compared to Sham group(P<0.05). CONCLUSIONS: Obvious damage of tissue flaps occurred after ischemia and reperfusion. Notch signaling pathway may be activated for tissue repairing and angiogenesis, and it may be induced by high level expression of HIF-1α under tissue hypoxia after ischemia-reperfusion injury.

Key words: Tissue flap, Ischemia-reperfusion injury, HIF-1α, Notch signaling pathway, Hypoxia

CLC Number:

R782.2

LI De-long, NIU Qi-fang, FENG Zhi-en, SU Ming, OUYANG Jia-jie, YANG Yang, LI Jin-zhong, XU Qiao-shi, HAN Zheng-xue.. Expression of HIF-1α and Notch-1 induced by hypoxia after ischemia reperfusion injury in tissue flap[J]. China Journal of Oral and Maxillofacial Surgery, 2018, 16(3): 209-214.

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