Intermittent nasal obstruction causes early effects on chondrogenesis of mandibular condylar chondrocytes

doi:10.19438/j.cjoms.2018.02.008

Abstract

Abstract: PURPOSE: In this study, we investigated the early effects of intermittent nasal obstruction on chondrogenesis of mandibular condylar chondrocytes (MCCs) via animal model. METHODS: Sixty four 4-week-old SD rats were randomly divided into 4 groups, each group contained 16 rats. Group A: control group; Group B: 4-day mouth breathing caused by nasal obstruction; Group C: 8-day mouth breathing caused by nasal obstruction; Group D: 16-day mouth breathing caused by nasal obstruction. The time of nasal obstruction was from 8:00 to 16:00 every day. Bilateral condylar MCCs were harvested and cultured in vitro, on 4th day, 8th day, 12th day and 16th day, respectively. Toluidine blue staining was used to identify MCCs,real-time polymerase chain reaction (RT-PCR) was used to detect the difference of gene expression of MCCs chondrogenic marker genes (SOX9,COL2a,ACAN,PTHrp). The data were statistically analyzed using SPSS19.0 software package. RESULTS: On 4th day of modeling, the expression of SOX9, COL2a and ACAN was lower in group B, C and D compared with group A (P<0.05); but the expression of PTHrp was higher in group B, C and D compared with group A (P<0.05). On 8th day of modeling, the expression of SOX9,COL2a,ACAN and PTHrp was higher in group B, C and D, but much higher in group B than in group C and D(P<0.05). On 12th day of modeling, group B, C and D showed a higher expression of PTHrp (P<0.05), and an equal or lower expression of COL2a, ACAN. On 16th day of modeling, the expression of COL2a, ACAN and PTHrp was increased in group C than in group A(P<0.05). CONCLUSIONS: It is suggested that during mouth breathing caused by nasal obstruction in young rats, the chondogenisis of MCCs decreased followed by a compensatory increase and finally decrease. Furthermore, early removal of nasal obstruction can cause a compensatory increase of MCCs chondrogenisis, but still causes a long-term negative influence.

Key words: Nasal obstruction, Mouth breathing, Mandibular, Mandibular condylar chondrocytes, MCCs, Chondrogenesis

CLC Number:

R782.2

SUN Hui-jun, WANG Xiao-ling, ZHU Yan-fei, YU Qian, NIE Ping, ZHU Min. Intermittent nasal obstruction causes early effects on chondrogenesis of mandibular condylar chondrocytes[J]. China Journal of Oral and Maxillofacial Surgery, 2018, 16(2): 138-143.

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