Inhibition of chitosan oligosaccharide on skull resorption in mice induced by lipopolysaccharides

doi:10.19438/j.cjoms.2018.02.001

Abstract

Abstract: PURPOSE: To study the effect of low molecular weight chitosan (low-molecular chitosan oligosaccharide, LMCOS) on lipopolysaccharides (LPS) -induced bone resorption in mice. METHODS: Six-week c57/bl6 male mice were randomly divided into 5 groups: phosphate buffer saline (PBS) group, LPS group, LPS+0.005% LMCOS group, LPS+0.05% LMCOS group and LPS+0.5% LMCOS group. There were 5 mice in each group, LPS was used at 10 mg/kg. After mild anesthesia, 300 μL corresponding reagents were injected into the skull bone. The mice were injected 3 times a week and sacrificed 2 weeks later. The skull bone was removed for micro-CT scan, H-E staining, TRAP staining. SPSS21.0 software package was used for statistical analysis. Bone damage area, osteoclast formation was observed and recorded. RESULTS: After LMCOS was added to LPS-induced skull resorption, bone destruction area decreased obviously; and with the increase of LMCOS concentration, bone destruction area and number of osteoclasts decreased gradually. CONCLUSIONS: LMCOS can inhibit the damage of skull bone in LPS-induced mice, which may have a therapeutic effect on osteolytic diseases.

Key words: Chitosan oligosaccharide, Lipopolysaccharides, Micro-CT, TRAP, Bone resorption

CLC Number:

R786.2

GUO Ke, HE Jia-jia, LIU Zong-lin, ZHANG Yao-sheng, WANG Wen-chao, WANG Ye-xin, XU Wei-feng, ZHANG Shan-yong. Inhibition of chitosan oligosaccharide on skull resorption in mice induced by lipopolysaccharides[J]. China Journal of Oral and Maxillofacial Surgery, 2018, 16(2): 97-101.

References

[1] Wieckiewicz M, Boening KW, Grychowska N, et al.Clinical application of chitosan in dental specialities[J]. Mini Rev Med Chem, 2017, 17(5): 401-409.
[2] Ramasamy P, Subhapradha N, Thinesh T, et al.Characterization of bioactive chitosan and sulfated chitosan from Doryteuthis singhalensis, (Ortmann, 1891)[J]. Int J Biol Macromol, 2017, 99: 682-691.
[3] Khodagholi F, Eftekharzadeh B, Maghsoudi N, et al.Chitosan prevents oxidative stress-induced amyloid β formation and cytotoxicity in NT2 neurons: involvement of transcription factors Nrf2 and NF-κB[J]. Mol Cell Biochem, 2010, 337(1-2): 39-51.
[4] Costa-Pinto AR, Correlo VM, Sol PC, et al.Osteogenic differentiation of human bone marrow mesenchymal stem cells seeded on melt based chitosan scaffolds for bone tissue engineering applications[J]. Biomacromolecules, 2009, 10(8): 2067-2073.
[5] Teng SH, Lee EJ, Wang P, et al.Three-layered membranes of collagen/ hydroxyapatite and chitosan for guided bone regeneration[J]. J Biomed Mater Res B Appl Biomater, 2010, 87(1): 132-138.
[6] Zhou T, Liu X, Sui B, et al.Development of fish collagen/bioactive glass/chitosan composite nanofibers as a GTR/GBR membrane for inducing periodontal tissue regeneration[J]. Biomed Mater, 2017, 12(5): 055004.
[7] 康献刚, 赵智远, 吴旭芝, 等. 壳聚糖-同种异体骨粉复合多孔支架修复大鼠骨缺损的实验研究[J]. 中国修复重建外科杂志, 2016, 30(3): 298-302.
[8] Li J, He J, Yu C.Chitosan oligosaccharide inhibits LPS-induced apoptosis of vascular endothelial cells through the BK Ca channel and the p38 signaling pathway[J]. Int J Mol Med, 2012, 30(1): 157-164.
[9] Xu Q, Wang W, Yang W, et al.Chitosan oligosaccharide inhibits EGF-induced cell growth possibly through blockade of epidermal growth factor receptor/mitogen-activated protein kinase pathway[J]. Int J Biol Macormol, 2017, 98: 502-505.
[10] Muanprasat C, Chatsudthipong V.Chitosan oligosaccharide: Biological activities and potential therapeutic applications[J]. Pharmacol Ther, 2017, 170: 80-97.
[11] Zhang C, Ling Y, Yan Z, et al.Chitosan oligosaccharides inhibit IL-1β-induced chondrocyte apoptosis via the P38 MAPK signaling pathway[J]. Glycoconj J, 2016, 33(5): 735-744.
[12] 文建华, 李真真, 贾伟建, 等. 胶原/壳寡糖复合凝胶对前成骨细胞MC3T3-E1s分化行为的影响[J]. 生物医学工程学杂志, 2016, 33(3): 493-498.
[13] Pelegrine AA, da Costa CE, Correa ME, et al. Clinical and histomorphometric evaluation of extraction sockets treated with an autologous bone marrow graft[J]. Clin Oral Implants Res, 2010, 21(5): 535-542.
[14] Khalifa AK, Wada M, Ikebe K, et al.To what extent residual alveolar ridge can be preserved by implant? A systematic review[J]. Int J Implant Dent, 2016, 2(1): 22-30.
[15] Kresnoadi U, Ariani MD, Djulaeha E, et al.The potential of mangosteen (Garcinia mangostana) peel extract, combined with demineralized freeze-dried bovine bone xenograft, to reduce ridge resorption and alveolar bone regeneration in preserving the tooth extraction socket[J]. J Indian Prosthodont Soc, 2017, 17(3): 282-288.
[16] Rasperini G, Canullo L, Dellavia C, et al.Socket grafting in the posterior maxilla reduces the need for sinus augmentation[J]. Int J Periodontics Restorative Dent, 2010, 30(3): 265-273.
[17] Maiorana C, Poli PP, Deflorian M, et al.Alveolar socket preservation with demineralised bovine bone mineral and a collagen matrix[J]. J Periodontal Implant Sci, 2017, 47(4): 194-210.
[18] Mayer Y, Zigdon-Giladi H, Machtei EE.Ridge preservation using composite alloplastic materials: a randomized control clinical and histological study in humans[J]. Clin Implant Dent Relat Res, 2016, 18(6): 1163-1170.
[19] 朱一博, 邱立新, 吴茴, 等. 应用骨胶原及富血小板纤维蛋白保存牙槽嵴[J]. 实用口腔医学杂志, 2014, 30(1): 70-73.
[20] Wang YF, Wang CY, Wan P, et al.Comparison of bone regeneration in alveolar bone of dogs on mineralized collagen grafts with two composition ratios of nano-hydroxyapatite and collagen[J]. Regen Biomater, 2016, 3(1): 33-40.
[21] Li L, Khansari A, Shapira L, et al.Contribution of interleukin-11 and prostaglandin(s) in lipopolysaccharide-induced bone resorption in vivo [J]. Infect Immun, 2002, 70(7): 3915-3922.
[22] 李箐, 于欣, 周钢, 等. 壳聚糖微球/小牛松质骨支架复合缓释体系的制备及体外生物活性评价[J]. 北京大学学报(医学版)医学版, 2016, 48(6):1043-1048.
[23] Jayash SN, Hashim NM, Misran M, et al.Formulation and in vitro and in vivo evaluation of a new osteoprotegerin-chitosan gel for bone tissue regeneration[J]. J Biomed Mater Res A, 2017, 105(2): 398-407.
[24] Ruan SQ, Deng J, Yan L, et al.Composite scaffolds loaded with bone mesenchymal stem cells promote the repair of radial bone defects in rabbit model[J]. Biomed Pharmacother, 2017, 97: 600-606.
[25] Teng SH, Lee EJ, Wang P, et al.Three-layered membranes of collagen/hydroxyapatite and chitosan for guided bone regeneration[J]. J Biomed Mater Res B Appl Biomater, 2008, 87(1): 132-138.
[26] Guo J, Yang D, Okamura H, et al.Calcium hydroxide suppresses Porphyromonas endodontalis lipopolysaccharide-induced bone destruction[J]. J Dent Res, 2014, 93(5): 508-513.
[27] Cao JJ, Gregoire BR, Shen CL.A high-fat diet decreases bone mass in growing mice with systemic chronic inflammation induced by low-dose, slow-release lipopolysaccharide pellets[J]. J Nutr, 2017, 147(10): 1909-1916.