Mechanism of maggot excretion/secretion in anti-intracellular Staphylococcus aureus internalization of osteoblast: an in vitro study

doi:10.19438/j.cjoms.2020.05.003

Abstract

Abstract: PURPOSE: This study was aimed to explore the mechanism of excretion/secretion (E/S) in protecting osteoblast being internalized by Staphylococcus aureus through in vitro experiments. METHODS: Excretion/secretion(E/S), E/S pre-treated with serine protease inhibitors(PMSF), E/S pre-treated with chymotrypsin inhibitors VR23 and PBS were respectively added to the mouse pre-osteoblast cell line (MC 3T3-E1) for culture. After 24, 48, 72 hours, Western blot was used to detect the fibronectin degradation, and CCK-8 was used to detect cell viability. After 24 hours, the cell line was co-cultured with Staphylococcus aureus to form intracellular bacteria to observe whether intracellular bacteria had formed in different groups. Flat colony counting method and immunofluorescence method were used to observe intracellular bacteria. The data were analyzed by SPSS 25.0 software package. RESULTS: E/S degraded fibronectin in extracellular matrix without influencing cell viability. Through flat colony counting method, the least intracellular bacteria were observed in the cell line treated with E/S. In cell line treated with E/S pre-treated with PMSF or the chymotrypsin protease inhibitor, more intracellular bacteria were observed. In PBS treated cell line, the most intracellular bacteria were observed. These results showed statistically significant(P<0.05) and similar results were obtained from immunofluorescence photography. CONCLUSIONS: E/S can be used to reduce the effect of Staphylococcus aureus on the internalization of MC 3T3-E1, which provides a new idea for the treatment of chronic refractory osteomyelitis.

Key words: Larva, Osteomyelitis, Staphylococcus aureus, Fibronectins, Intracellular bacteria

CLC Number:

R782.3

ZHU Fang-xing, XU Xiao-feng, SI Jia-wen, LIAO Qian, XU Bing. Mechanism of maggot excretion/secretion in anti-intracellular Staphylococcus aureus internalization of osteoblast: an in vitro study[J]. China Journal of Oral and Maxillofacial Surgery, 2020, 18(5): 395-400.

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