叶黄素对兔创伤性颞下颌关节强直的预防作用研究

doi:10.19438/j.cjoms.2024.03.002

摘要/Abstract

摘要： 目的：探讨叶黄素对兔创伤性颞下颌关节强直的作用。方法：将16只新西兰大白兔随机分为空白对照组及叶黄素干预组（实验组）,所有实验动物均在保护关节囊的前提下建立创伤性颞下颌关节强直模型。术后实验组口服叶黄素10 mg·kg^-1·d^-1,连续给药4周。比较2组动物的最大开口度、体重、组织学、影像学、骨代谢指标等方面的差异。采用GraphPad Prism 9.4.0软件包对数据进行统计学分析。结果：术后实验组动物体重、开口度、血清骨钙素浓度、CT测量的髁突表面积及体积显著高于对照组（P<0.05）。组织形态学观察显示,2组实验动物髁突残端可见编织骨和新生骨,纤维中有时可见软骨化生灶,偶可见肉芽组织和陈旧性出血。实验组髁突纤维层增厚,细胞增殖活跃,细胞层次更加明显。结论：叶黄素对创伤性颞下颌关节强直的发生有一定预防作用,其作用机制可能与叶黄素促进骨形成、提高血清骨钙素浓度等有关。

关键词: 叶黄素, 创伤性颞下颌关节强直, 动物模型, 兔

Abstract: PURPOSE: To explore the effect of lutein on traumatic temporomandibular joint ankylosis. METHODS: Sixteen New Zealand white rabbits were randomly divided into blank control group and lutein intervention group (experimental group). The traumatic temporomandibular joint ankylosis model was established in all experimental animals under the premise of protecting the joint capsule. Postoperatively, the experimental group was given oral lutein 10 mg·kg^-1·d^-1 for 4 consecutive weeks. The maximum mouth opening, body weight, histology, imaging and bone metabolism indexes were compared between the two groups. GraphPad Prism 9.4.0 software package was used for data analysis. RESULTS: The body weight, mouth opening, serum osteocalcin concentration, condylar surface area and volume measured by CT in the experimental group were significantly higher than those in the control group after operation(P<0.05). Histomorphological observation showed that braid bone and new bone could be seen at the condylar stump of the two groups of experimental animals, sometimes chondrometaplasia could be seen in the fibers, occasionally granulation tissue and long-standing bleeding could be seen. The experimental group had thickening fibrous layer of the condyle, active cell proliferation, and a more pronounced cellular hierarchy. CONCLUSIONS: Lutein has certain preventive effect on traumatic temporomandibular joint ankylosis, and its mechanism of action may be related to its ability to promote bone formation and increase serum osteocalcin concentration.

Key words: Lutein, Traumatic temporomandibular joint ankylosis, Animal models, Rabbit

中图分类号:

R782.6

米娜, 安玮, 金雪梅, 谢帅, 姚志涛. 叶黄素对兔创伤性颞下颌关节强直的预防作用研究[J]. 中国口腔颌面外科杂志, 2024, 22(3): 216-221.

MI Na, AN Wei, JIN Xue-mei, XIE Shuai, YAO Zhi-tao. Research on the preventive effect of lutein on traumatic temporomandibular joint ankylosis of rabbits[J]. China J Oral Maxillofac Surg, 2024, 22(3): 216-221.

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