糖尿病兔骨髓间充质干细胞成骨分化能力探讨

doi:10.19438/j.cjoms.2017.02.002

摘要/Abstract

摘要： 目的观察糖尿病兔骨髓间充质干细胞（bone arrow-derived mesenchymal stem cells,BMSCs）以成骨分化为主的生物学特性。方法通过静脉注射水合四氧嘧啶,建立糖尿病模型,以注射等量生理盐水作为对照组。在无菌条件下,局麻后抽取兔髂骨骨髓,获取BMSCs。采用全骨髓贴壁培养法对细胞进行纯化及培养,分别对2组细胞行MTT检测、实时定量PCR检测、碱性磷酸酶（ALP）活性检测,以及ERK、AKT信号通路表达检测。采用SPSS 13.0软件包对数据进行单因素方差分析（ANOVA）和SNK post hoc分析。结果与正常组比较,实验组糖尿病兔BMSCs细胞增殖活性、成骨基因表达、ALP活性及成血管基因表达均显著下降（P<0.05）。结论糖尿病兔BMSCs的增殖活性、成骨分化能力及成血管基因表达能力均受到损害。

关键词: 糖尿病兔, 骨髓间充质干细胞, 增殖活性, 成骨分化, 成血管分化

Abstract: PURPOSE : To observe the biological characteristics (mainly osteogenesis ability) of bone marrow-derived mesenchymal stem cells (BMSCs) in diabetic rabbits. METHODS : Diabetic rabbit models were established by injecting alloxan intravenously, which were used as the experimental group, rabbits receiving normal saline injection were served as the control group. Rabbit iliac bone marrow was extracted under aseptics and local anesthesia, and BMSCs were isolated and cultured by using whole blood adherent culture method. MTT assay, real-time PCR, and alkaline phosphatase (ALP) staining of the BMSCs in both groups were performed, respectively. Differences between groups were analyzed with SPSS 13.0 software package for ANOVA and SNK post hoc analysis. RESULTS : Compared with the control group, the ability of cell proliferation, osteogenic gene expression, ALP activity and angiogenic gene expression of the diabetic rabbits BMSCs were all decreased, and the difference was statistically significant (P<0.05). CONCLUSION S: The activity of cell proliferation, osteogenesis and angiogenesis of the diabetic rabbit BMSCs is damaged.

Key words: Diabetic rabbit, BMSCs, Proliferation activity, Osteogenesis, Angiogenesis

中图分类号:

R329.2

周宇宁, 马吴迪, 桂志鹏, 夏伦果, 徐袁瑾. 糖尿病兔骨髓间充质干细胞成骨分化能力探讨[J]. 中国口腔颌面外科杂志, 2017, 15(2): 103-108.

ZHOU Yu-ning, MA Wu-di, GUI Zhi-peng, XIA Lun-guo, XU Yuan-jin. Osteogenesis ability of bone marrow-derived mesenchymal stem cells derived from diabetic rabbits[J]. China J Oral Maxillofac Surg, 2017, 15(2): 103-108.

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