口腔鳞状细胞癌组织中miR-155-5p、miR-138及miR-381-3p的表达变化及临床意义

doi:10.19438/j.cjoms.2025.02.009

摘要/Abstract

摘要： 目的：探讨口腔鳞状细胞癌（oral squamous cell carcinoma,OSCC）组织中微小核糖核酸-155-5p（miR-155-5p）、miR-138、miR-381-3p的表达变化及临床意义。方法：收集手术切除的72例OSCC患者的癌组织及癌旁正常组织。采用实时荧光定量PCR（qPCR）检测miR-155-5p、miR-138、miR-381-3p mRNA的表达,分析OSCC组织中miR-155-5p、miR-138、miR-381-3p表达与临床病理特征及预后的关系。采用SPSS 25.0软件包对数据进行统计学分析。结果：与癌旁正常组织相比,癌组织中miR-155-5p显著升高,miR-138、miR-381-3p表达显著降低（P<0.05）。肿瘤最大径≥3 cm、肿瘤浸润深度≥5 mm、临床Ⅲ~Ⅳ分期、颈淋巴结转移、中高分化患者癌组织中呈现miR-155-5p mRNA高表达及miR-138、miR-381-3p mRNA低表达（P<0.05）。随访2年,72例OSCC患者生存率为40.32%（25/72）,与生存患者相比,死亡患者的miR-155-5p mRNA表达量更高,miR-138、miR-381-3p mRNA表达量更低。受试者工作特征（receiver operating characteristic,ROC）曲线分析显示,miR-155-5p、miR-138、miR-381-3p 联合预测口腔鳞状细胞癌患者预后不良的曲线下面积为0.957,均高于单一指标检测（P<0.05）。结论：OSCC组织中miR-155-5p呈现高表达,miR-138、miR-381-3p 呈低表达,三者表达情况与肿瘤的侵袭深度、临床分期、颈淋巴结转移、分化程度及预后有关,临床上可联合3项指标检测,评估患者预后。

关键词: 口腔鳞状细胞癌, miR-155-5p, miR-138, miR-381-3p, 临床病理特征, 预后

Abstract: PURPOSE: To investigate the expression and clinical value of microRNA-155-5p (miR-155-5p), miR-138 and miR-381-3p in oral squamous cell carcinoma(OSCC). METHODS: Cancer tissues and adjacent normal tissues of 72 patients with OSCC were collected. Quantitative real-time PCR(RT-PCR) was used to detect the mRNA expressions of miR-155-5p, miR-138 and miR-381-3p, and to analyze the relationship between the expressions of miR-155-5p, miR-138 and miR-381-3p and clinicopathological characteristics and prognosis in OSCC. SPSS 25.0 software package was used for data analysis. RESULTS: Compared with adjacent normal tissues, the expression of miR-155-5p in cancer tissues was significantly increased, and the expression of miR-138 and miR-381-3p was significantly decreased(P< 0.05). High expression of miR-155-5p mRNA and low expression of miR-138 and miR-381-3p mRNA were found in cancer tissues of patients with tumor maximum diameter ≥ 3 cm, tumor invasion depth ≥ 5 mm, clinical stage Ⅲ-Ⅳ, cervical lymph node metastasis and moderate to high differentiation(P< 0.05). After 2 years of follow-up, the survival rate of 72 patients with OSCC was 40.32%(25/72). Compared with the survival patients, the expression of miR-155-5p mRNA in the dead patients was higher, and the expression of miR-138 and miR-381-3p mRNA was lower. Receiver operating characteristic (ROC) analysis showed that the area under the curve(AUC) of miR-155-5p, miR-138 and miR-381-3p combined to predict poor prognosis in patients with OSCC was 0.957, which was significantly higher than that of single mRNA detection (P< 0.05). CONCLUSIONS: The expression of miR-155-5p in OSCC tissues is high, while miR-138 and miR-381-3p expression are low. The expression of the three factors are related to the invasion depth, clinical stage, cervical lymph node metastasis, differentiation degree and prognosis of the tumor. Clinically, combined detection of the three indicators can be strengthened to evaluate the prognosis of patients.

Key words: Oral squamous cell carcinoma, miR-155-5p, miR-138, miR-381-3p, Clinicopathological features, Prognosis

中图分类号:

R739.8

王欣, 金城, 崔焕焕. 口腔鳞状细胞癌组织中miR-155-5p、miR-138及miR-381-3p的表达变化及临床意义[J]. 中国口腔颌面外科杂志, 2025, 23(2): 159-164.

WANG Xin, JIN Cheng, CUI Huan-huan. Expression changes and clinical value of miR-155-5p, miR-138, miR-381-3p in oral squamous cell carcinoma[J]. China J Oral Maxillofac Surg, 2025, 23(2): 159-164.

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