中国口腔颌面外科杂志

中国口腔颌面外科杂志 ›› 2020, Vol. 18 ›› Issue (4): 308-313.doi: 10.19438/j.cjoms.2020.04.004

• 论著 • 上一篇    下一篇

可利霉素对口腔鳞癌细胞生物学活性的影响

梁思源, 赵铜超, 周知航, 琚梧桐, 刘莹, 张志愿, 钟来平   

  1. 上海交通大学医学院附属第九人民医院·口腔医学院 口腔颌面-头颈肿瘤科,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011
  • 收稿日期:2019-12-30 出版日期:2020-07-20 发布日期:2020-09-10
  • 通讯作者: 钟来平,E-mail：zhonglaiping@163.com
  • 作者简介:梁思源（1995-）,女,在读硕士研究生,E-mail：liangsy0414@163.com
  • 基金资助:
    上海交通大学医学院附属第九人民医院临床研究型MDT项目（201916）; 上海交通大学医学院双百人计划（20191915）

Inhibitory effect of carrimycin on biological activity of oral squamous cell carcinoma cells in vitro

LIANG Si-yuan, ZHAO Tong-chao, ZHOU Zhi-hang, JU Wu-tong, LIU Ying, ZHANG Zhi-yuan, ZHONG Lai-ping   

  1. Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Reasearch Institute of Stomatology. Shanghai 200011, China
  • Received:2019-12-30 Online:2020-07-20 Published:2020-09-10

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摘要： 目的: 体外评价可利霉素对口腔鳞状细胞癌的抗肿瘤活性。方法: 梯度浓度可利霉素分别处理口腔鳞状细胞癌细胞（HN6/HB96细胞系）,采用CCK-8试剂盒检测细胞增殖,绘制生长曲线;采用划痕实验分析细胞迁移能力;采用平板克隆形成实验分析细胞克隆形成能力;采用流式细胞术分析细胞周期和细胞凋亡。采用 SPSS 18.0 软件包对数据进行统计学分析。结果: 可利霉素浓度依赖性抑制HN6/HB96的体外增殖,随着药物浓度增加,细胞迁移和克隆形成能力显著下降（P<0.05）;可利霉素使处理过的口腔鳞状细胞癌细胞G1期比例显著升高,S期和G2/M期比例显著下降,细胞周期阻滞于G1期（P<0.001）。可利霉素诱导口腔鳞状细胞癌细胞凋亡,随着药物浓度增加,细胞凋亡率显著上升（P<0.01）。结论: 可利霉素体外抑制口腔鳞状细胞癌细胞的生物学活性,为可利霉素用于口腔鳞状细胞癌的治疗提供了实验依据。

关键词: 可利霉素, 抗生素, 口腔鳞状细胞癌

Abstract: PURPOSE: As a new macrolide antibiotic with independent intellectual property rights of China, carrimycin not only has good antibacterial effect on a variety of bacteria, but also has the function of regulating cell physiology and proliferation.The aim of this study was to investigate the anti-tumor effects of carrimycin on the biological activity of oral squamous cell carcinoma (OSCC) cells in vitro. METHODS: OSCC cells (HN6/HB96 cell lines) were treated with gradient concentration of carrimycin. Cell proliferation was detected using CCK-8 kit, based on which cell growth curve was drawn. Cell migration ability was analyzed by wound-healing assay. Cell clone formation ability was analyzed by clone formation assay. Cell cycle and apoptosis were analyzed by flow cytometry. SPSS18.0 software package was used for statistical analysis. RESULTS: Carrimycin concentration-dependently inhibited cellular proliferation of OSCC cells of HN6 and HB96 in vitro. With the increase of drug concentration, cell migration and clonal formation ability significantly decreased (P<0.05). The G1 phase ratio of OSCC cells treated with carrimycin significantly increased, while the S and G2/M phase ratio decreased, indicating that the cell cycle was arrested in G1 phase(P<0.001). The apoptosis rate of OSCC cells was significantly increased with the increase of drug concentration (P<0.01). CONCLUSIONS: The activity of OSCC cells can be effectively inhibited by carrimycin, which provides experimental evidence for the application of carrimycin in OSCC.

Key words: Carrimycin, Antibiotics, Oral squamous cell carcinoma

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