中国口腔颌面外科杂志

中国口腔颌面外科杂志 ›› 2019, Vol. 17 ›› Issue (4): 289-294.doi: 10.19438/j.cjoms.2019.04.001

• 论著 • 上一篇    下一篇

沉默环状RNA hsa_circ_0006677 表达对口腔鳞状细胞癌迁移及侵袭能力的影响

李翔1,2,3, 苏文1,2,3, 张寒雨1,3, 张璧茹1,3, 杨宏宇1,2,3   

  1. 1.安徽医科大学北京大学深圳医院临床学院,广东 深圳 518036;
    2.安徽医科大学,安徽 合肥 230032;
    3.北京大学深圳医院 口腔颌面外科,广州 深圳 518036
  • 收稿日期:2019-01-21 修回日期:2019-04-05 出版日期:2019-07-20 发布日期:2019-08-12
  • 通讯作者: 杨宏宇,E-mail: hyyang192@hotmail.com
  • 作者简介:李翔（1991-）,男,在读硕士研究生,E-mail:280177530@qq.com
  • 基金资助:
    国家自然科学基金（81572654）; 深圳市科技创新基础研究基金（JCYJ201504091443303、JCYJ20150404091443286、JCYJ2016042873933559、SZBC2017023）; 深圳市三名工程项目（SZSM 201512036）

Effect of silencing circular RNA hsa_circ_0006677 on migration and invasion of oral squamous cell carcinoma

LI Xiang1,2,3, SU Wen1,2,3, ZHANG Han-yu1,3, ZHANG Bi-ru1,3, YANG Hong-yu1,2,3   

  1. 1.Clinical School, Peking University Shenzhen Hospital, Anhui Medical University. Shenzhen 518036, Guangdong Province;
    2.Anhui Medical University. Hefei 230032, Anhui Province;
    3.Department of Oral and Maxillofacial Surgery, Peking University Shenzhen Hospital. Shenzhen 518036, Guangdong Province, China
  • Received:2019-01-21 Revised:2019-04-05 Online:2019-07-20 Published:2019-08-12

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摘要： 目的 研究hsa_circ_0006677在口腔鳞状细胞癌（oral squamous cell carcinoma, OSCC）中的表达及其对OSCC迁移和侵袭能力的影响。方法 使用定量PCR（qRT-PCR）在40对配对的OSCC组织样品中验证hsa_circ_0006677的相对表达量;通过受试者工作特征曲线（receiver operating characteristic curve,ROC）,分析hsa_circ_0006677的相对表达与临床信息的相关性。利用qRT-PCR实验检测hsa_circ_0006677在口腔鳞癌细胞系（SCC-9、SCC-15、SCC-25、CAL-27）及人口腔角化细胞（HOK）的相对表达量,划痕实验和Transwell实验检测沉默hsa_circ_0006677对口腔鳞癌细胞SCC-15细胞侵袭和迁移能力的影响。采用Graphpad 5.0软件对数据进行统计学分析。结果 hsa_circ_0006677在OSCC组织中与配对的正常组织相比显著下调（P<0.001）,hsa_circ_0006677的相对表达与淋巴结转移相关（P=0.0405）。ROC曲线下面积（AUC）为0.8221,cutoff值为10.74,灵敏度和特异度分别为0.8095和0.7619。qRT-PCR检测hsa_circ_0006677在SCC-15和CAL-27内相对表达量明显降低;划痕实验和Transwell实验显示,在SCC-15细胞内沉默hsa_circ_0006677后,其转移能力和侵袭能力显著提高。结论 口腔鳞癌中环状RNA hsa_circ_0006677在OSCC中明显下调,沉默hsa_circ_0006677可明显促进OSCC的转移和侵袭能力。

关键词: 环状RNA, hsa_circ_0006677, 口腔鳞状细胞癌, 转移, 侵袭

Abstract: PURPOSE: To investigate the role of aberrantly expressed hsa_circ_0006677 in oral squamous cell carcinoma (OSCC) and its effect on OSCC migration and invasion. Methods: Quantitative PCR (qRT-PCR) was used to validate hsa_circ_0006677 in 40 pairs of matched OSCC tissue samples. The correlation between hsa_circ_0006677 expression and clinical information and receiver operating characteristic (ROC) curve was analysed. qRT-PCR assay was used to detect the expression of hsa_circ_0006677 in OSCC cell lines (SCC-9, SCC-15, SCC-25, CAL-27) and human oral keratinocytes (HOK). Scratch test and Transwell assay were used to evaluate the effect of silencing hsa_circ_0006677 on invasion and migration of OSCC cells SCC-15. The data were statistically analysed with Graphpad 5.0 software. Results: Hsa_circ_0006677 was significantly down-regulated in OSCC tissues compared with matched normal tissues (P<0.001), and the relative expression of hsa_circ_0006677 was correlated with cervical lymph node metastasis (P= 0.0405). The area under ROC curve (AUC) was 0.8221. The cutoff value was 10.74, the sensitivity and specificity were 0.8095 and 0.7619, respectively. The expression of hsa_circ_0006677 in SCC-15 and CAL-27 was significantly decreased. After transfecting si-hsa_circ_0006677 into SCC-15 cells, the ability of invasion and metastasis was significantly improved. Conclusions: The circular RNA hsa_circ_0006677 is significantly down-regulated in OSCC. Silencing hsa_circ_0006677 can significantly promote metastasis and invasion of SCC-15 cells.

Key words: Circular RNA, Hsa_circ_0006677, Oral squamous cell carcinoma, Metastasis, Invasion

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