巨噬细胞来源的白细胞介素6对口腔鳞癌细胞增殖活性的影响

doi:10.19438/j.cjoms.2019.06.004

中国口腔颌面外科杂志 ›› 2019, Vol. 17 ›› Issue (6): 503-507.doi: 10.19438/j.cjoms.2019.06.004

巨噬细胞来源的白细胞介素6对口腔鳞癌细胞增殖活性的影响

李捷, 陈巨峰, 冼淡

佛山市第一人民医院口腔科,广东佛山 528000

收稿日期:2019-04-17 出版日期:2019-11-20 发布日期:2019-12-16
通讯作者: 李捷,E-mail:betty-chow@163.com
作者简介:李捷（1974-）,男,大专,主治医师

Macrophage-derived IL-6 promotes oral cancer cell proliferation

LI Jie, CHEN Ju-feng, XIAN Dan

Department of Stomatology, The First People′s Hospital of Foshan. Foshan 528000, Guangdong Province, China

Received:2019-04-17 Online:2019-11-20 Published:2019-12-16

摘要/Abstract

摘要： 目的: 探讨巨噬细胞来源的白细胞介素6（interleukin 6,IL-6）对口腔鳞状细胞癌细胞系增殖活性的影响。方法: 通过MTT法检测不同浓度重组IL-6对口腔鳞状细胞癌细胞系SCC-15和CAL-27增殖活性的影响。建立佛波酯诱导的THP-1巨噬细胞分化模型,分别通过实时定量荧光PCR（RT-PCR）和酶联免疫吸附试验,分析脂多糖（lipopolysaccharide,LPS）刺激下的THP-1细胞中mRNA水平的变化以及THP-1细胞培养基中IL-6的含量。利用MTT法检测含THP-1条件培养基对SCC-15和CAL-27细胞系增殖活性的影响。采用GraphPad 7.0软件包对数据进行统计学分析。结果: 培养基中添加IL-6,可促进SCC-15和CAL-27细胞系的增殖活性。LPS可刺激活化的THP-1巨噬细胞分泌IL-6;活化的THP-1细胞培养基可促进SCC-15和CAL-27细胞的增殖活性。结论: LPS刺激巨噬细胞分泌的IL-6,可促进口腔鳞状细胞癌细胞系SCC-15和CAL-27的增殖活性。

关键词: 巨噬细胞, 白细胞介素6, 口腔癌, 增殖

Abstract: PURPOSE: To investigate the effect of macrophage-derived IL-6 on oral cancer cell proliferation. METHODS: The proliferation rates of SCC-15 and CAL-27 cells under different concentration of IL-6 were measured by MTT assay. THP-1 macrophages were induced by PMA, IL-6 mRNA level in LPS-stimulated THP-1 cells were measured by real-time qPCR, IL-6 protein contents in THP-1 conditional medium were measured by ELISA. SCC-15 and CAL-27 cells were co-cultured with THP-1 conditional medium, cell proliferation rate was measured by MTT assay. The data were analyzed by GraphPad 7.0 software package. RESULTS: Recombinant IL-6 in culture medium promoted SCC-15 and CAL-17 cell proliferation; LPS stimulated IL-6 expression in PMA-induced THP-1 macrophages. THP-1 conditional medium promoted SCC-15 and CAL-27 cell proliferation. CONCLUSIONS: IL-6 derived by LPS-stimulated macrophages could promote SCC-15 and CAL-27 cell proliferation.

Key words: Macrophage, Interleukin 6, Oral cancer, Proliferation

中图分类号:

R739.8

李捷, 陈巨峰, 冼淡. 巨噬细胞来源的白细胞介素6对口腔鳞癌细胞增殖活性的影响[J]. 中国口腔颌面外科杂志, 2019, 17(6): 503-507.

LI Jie, CHEN Ju-feng, XIAN Dan. Macrophage-derived IL-6 promotes oral cancer cell proliferation[J]. China J Oral Maxillofac Surg, 2019, 17(6): 503-507.

参考文献

[1] Principe S, Hui AB, Bruce J, et al.Tumor-derived exosomes and microvesicles in head and neck cancer: implications for tumor biology and biomarker discovery[J]. Proteomics, 2013, 13(10-11): 1608-1623.
[2] Plzak J, Boucek J, Bandurova V, et al.The head and neck squamous cell carcinoma microenvironment as a potential target for cancer therapy[J]. Cancers (Basel), 2019, 11(4): E440.
[3] Cassetta L, Pollard JW.Targeting macrophages: therapeutic approaches in cancer[J]. Nat Rev Drug Discov, 2018, 17(12):887-904.
[4] Song W, Mazzieri R, Yang T, et al.Translational significance for tumor metastasis of tumor-associated macrophages and epithelial-mesenchymal transition[J]. Front Immunol, 2017, 8: 1106.
[5] Lotfi A, Shahidi N, Bayazian G, et al.Serum level of interleukin-6 in patients with oral tongue squamous cell carcinoma[J]. Iran J Otorhinolaryngol, 2015, 27(80): 207-211.
[6] Lisa Cheng YS, Jordan L, Gorugantula LM, et al.Salivary interleukin-6 and -8 in patients with oral cancer and patients with chronic oral inflammatory diseases[J]. J Periodontol, 2014, 85(7): 956-965.
[7] St John MA, Li Y, Zhou X, et al.Interleukin 6 and interleukin 8 as potential biomarkers for oral cavity and oropharyngeal squamous cell carcinoma[J]. Arch Otolaryngol Head Neck Surg, 2004, 130(8): 929-935.
[8] Korn T, Bettelli E, Oukka M, et al.IL-17 and Th17 Cells[J]. Annu Rev Immunol, 2009, 27: 485-517.
[9] Bettelli E, Carrier Y, Gao W, et al.Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cells[J]. Nature, 2006, 441(7090): 235-238.
[10] Taniguchi K, Karin M.IL-6 and related cytokines as the critical lynchpins between inflammation and cancer[J]. Semin Immunol, 2014, 26(1): 54-74.
[11] Mauer J, Denson JL, Bruning JC.Versatile functions for IL-6 in metabolism and cancer[J]. Trends Immunol, 2015, 36(2): 92-101.
[12] Johnson DE, O'keefe RA, Grandis J R. Targeting the IL-6/JAK/STAT3 signalling axis in cancer[J]. Nat Rev Clin Oncol, 2018, 15(4): 234-248.
[13] Genin M, Clement F, Fattaccioli A, et al.M1 and M2 macrophages derived from THP-1 cells differentially modulate the response of cancer cells to etoposide[J]. BMC Cancer, 2015, 15: 577.
[14] Rey-Giraud F, Hafner M, Ries CH.In vitro generation of monocyte-derived macrophages under serum-free conditions improves their tumor promoting functions[J]. PLoS One, 2012, 7(8): e42656.
[15] Zhao Y, Adjei AA.Targeting angiogenesis in cancer therapy: moving beyond vascular endothelial growth factor[J]. Oncologist, 2015, 20(6): 660-673.

巨噬细胞来源的白细胞介素6对口腔鳞癌细胞增殖活性的影响

Macrophage-derived IL-6 promotes oral cancer cell proliferation

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	陈华, 李婧, 罗梅. 红景天甙通过NRF2/KEAP1通路对口腔鳞癌细胞生长的影响[J]. 中国口腔颌面外科杂志, 2024, 22(5): 424-429.
[2]	赵安玉琢, 石兴莲, 刘欢, 姜健红, 李哲臻, 刘梅. 人工智能在口腔癌早期诊断中的应用研究进展[J]. 中国口腔颌面外科杂志, 2024, 22(5): 493-498.
[3]	高鹏飞, 林洁. 区域神经阻滞技术用于口腔癌缺损皮瓣修复手术的研究进展[J]. 中国口腔颌面外科杂志, 2024, 22(4): 400-403.
[4]	惠琪, 高万鹏, 李欢, 赵琦, 王珺, 魏建华, 杨新杰, 杨子桧. 唾液腺腺样囊性癌微环境中肿瘤相关巨噬细胞外泌体差异表达miRNAs的筛选及验证[J]. 中国口腔颌面外科杂志, 2024, 22(3): 209-215.
[5]	洪磊, 陈伟良. 口腔癌老年患者术后静脉血栓栓塞症的危险因素分析[J]. 中国口腔颌面外科杂志, 2024, 22(3): 233-238.
[6]	王帅, 何杏芳, 王蕴洁, 吴伟勤, 黄秋雨. 非药物干预对口腔癌患者术后谵妄预防效果的系统评价[J]. 中国口腔颌面外科杂志, 2024, 22(3): 279-286.
[7]	周新凯¹,张月影¹,滕晓菊²,邹卫珍²,吴瑞芳². 口腔癌患者二元应对现状及影响因素分析[J]. 中国口腔颌面外科杂志, 2024, 22(2): 170-174.
[8]	李华盛, 周迪, 韩楠男, 严明, 阮敏. 石蒜碱通过降解SCAP蛋白抑制口腔鳞癌细胞增殖与侵袭的实验研究[J]. 中国口腔颌面外科杂志, 2024, 22(1): 29-35.
[9]	陈瑞斌, 陈岚枫. 2011—2017年福建省口腔癌发病及死亡趋势分析[J]. 中国口腔颌面外科杂志, 2024, 22(1): 68-72.
[10]	林惠, 高云飞, 刘晶, 马旭辉. 槐角提取物对颌骨骨髓间充质干细胞成骨向分化的影响[J]. 中国口腔颌面外科杂志, 2024, 22(1): 73-77.
[11]	陈歆旖, 李晨蕾, 秦兴军, 张金凤. 口腔癌患者营养状况变化轨迹及其影响因素分析[J]. 中国口腔颌面外科杂志, 2023, 21(6): 538-547.
[12]	周新凯, 滕晓菊, 张月影. 基于CiteSpace口腔癌患者生存质量研究的可视化分析[J]. 中国口腔颌面外科杂志, 2023, 21(6): 586-592.
[13]	许贵松, 杨功鑫, 游元和, 田卓炜, 肖孟, 杜仲, 王延安. 20例面部先天性浸润性脂肪增殖症的临床及影像学分析[J]. 中国口腔颌面外科杂志, 2023, 21(5): 472-478.
[14]	陈玉, 陶明, 雷银富, 吴和梅, 彭晓峰, 苟登群. 口腔癌患者术后肺部感染危险因素的meta分析[J]. 中国口腔颌面外科杂志, 2023, 21(5): 511-517.
[15]	李贵忠, 李洁莹, 周凯, 孟宇翔, 王可心, 葛胜优, 宋凯, 冯元勇, 陶月琴, 展晓红, 尚伟. 淋巴结外扩展对口腔鳞癌患者TNM分期及生存预后的影响[J]. 中国口腔颌面外科杂志, 2023, 21(4): 377-383.