红景天甙通过NRF2/KEAP1通路对口腔鳞癌细胞生长的影响

doi:10.19438/j.cjoms.2024.05.002

摘要/Abstract

摘要： 目的: 探讨红景天甙对口腔鳞癌细胞生长的影响及其机制。方法: 以40 μg/mL（低剂量组）、60 μg/mL（中剂量组）、100 μg/mL（高剂量组）红景天甙处理WSU-HN30细胞,等体积培养液处理作为空白对照组。分别通过MTT法、Transwell实验及流式细胞术检测WSU-HN30细胞增殖、迁移及凋亡,通过实时定量PCR（RT-PCR）和Western免疫印迹法检测核因子E2相关因子2（NRF2）和Kelch样ECH相关蛋白1（KEAP1）mRNA和蛋白的表达。采用SPSS 20.0软件包对数据进行统计学分析。结果: 同一浓度红景天甙作用WSU-HN30细胞48 h后,增殖抑制率显著高于24 h（P<0.05）;同一时间,随着红景天甙浓度增加,增殖抑制率显著增加（P<0.05）;与空白对照组相比,红景天甙处理后细胞迁移数显著减少,细胞凋亡率显著增加,细胞NRF2和KEAP1 mRNA及蛋白相对表达量显著降低,呈浓度依赖性（P<0.05）。结论: 红景天甙可显著抑制口腔鳞癌细胞增殖和迁移,诱导细胞凋亡,其机制与下调NRF2/KEAP1通路表达有关。

关键词: 口腔鳞癌, 红景天甙, NRF2/KEAP1通路, 增殖, 迁移, 凋亡

Abstract: PURPOSE: To investigate the effect and mechanism of salidroside on the growth of oral squamous cell carcinoma cells. METHODS: WSU-HN30 cells were divided into three salidroside treatment groups, low-dose group(40 μg/mL), middle-dose group(60 μg/mL) and high-dose group(100 μg/mL). The proliferation, migration and apoptosis of WSU-HN30 cells were detected by MTT assay, Transwell assay and flow cytometry, and the mRNA and protein expressions of nuclearfactor erythroidderived 2-like 2 (NRF2) and kelch-like ECH associated protein 1 (KEAP1) were detected by real-time quantitative PCR(RT-PCR) and Western blot. SPSS 20.0 software package was used for data analysis. RESULTS: At the same concentration, the proliferation inhibition rate of salidroside after 48 h was significantly higher than that after 24 h (P<0.05). At the same time, with the salidroside concentration increasing, the proliferation inhibition rate was significantly increased(P<0.05). Compared with blank control group, salidroside treatment groups had significant decrease in the number of cell migration, significant increase in the rate of apoptosis, and significant decrease in mRNA and protein expressions of NRF2 and KEAP1; Moreover, the above changes showed a concentration-dependent manner(P<0.05). CONCLUSIONS: Salidroside can significantly inhibit the proliferation and migration of oral squamous cell carcinoma cells, and induce cell apoptosis, and its mechanism may be related to the down-regulation of expression of NRF2/KEAP1 pathway.

Key words: Oral squamous cell carcinoma, Salidroside, NRF2/KEAP1 pathway, Proliferation, Migration, Apoptosis

中图分类号:

R739.8

陈华, 李婧, 罗梅. 红景天甙通过NRF2/KEAP1通路对口腔鳞癌细胞生长的影响[J]. 中国口腔颌面外科杂志, 2024, 22(5): 424-429.

CHEN Hua, LI Jing, LUO Mei. Effect of salidroside on the growth of oral squamous cell carcinoma cells through regulating NRF2/KEAP1 pathway[J]. China J Oral Maxillofac Surg, 2024, 22(5): 424-429.

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