点阵Er:YAG激光照射兔口腔黏膜后的组织学变化

中国口腔颌面外科杂志 ›› 2015, Vol. 13 ›› Issue (2): 117-123.

点阵Er:YAG激光照射兔口腔黏膜后的组织学变化

田海锁^{1, 2}, 蔺栋鹏¹, 赵天一¹, 于擘^{1, *}, 马秦^{1, *}

1.第四军医大学附属口腔医院口腔颌面外科,军事口腔医学国家重点实验室,陕西西安 710032;
2.武警辽宁省总队医院口腔科,辽宁沈阳 110034

收稿日期:2014-09-25 修回日期:2014-11-27 出版日期:2015-03-20 发布日期:2015-04-23
通讯作者: 于擘,Tel:029-84772533,E-mail:yuboli@fmmu.edu.cn; 马秦,Tel:029-84772531,E-mail:qinma@fmmu.edu.cn。*共同通信作者
作者简介:田海锁（1977-）,男,硕士,主治医师,E-mail: 80604798@qq.com
基金资助:
陕西省科技计划项目（2012SF2-20-2）

Histological change of rabbit oral mucosa after fractional Er:YAG laser irradiation

TIAN Hai-suo^{1, 2}, LIN Dong-peng¹, ZHAO Tian-yi¹, YU Bo¹, MA Qin¹

1.State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Forth Military Medical University. Xi'an 710032, Shaanxi Province;
2. Department of Stomatology, Armed Police Corps Hospital in Liaoning Province. Shenyang 110034, Liaoning Province, China

Received:2014-09-25 Revised:2014-11-27 Online:2015-03-20 Published:2015-04-23
Supported by:
Supported by Science and Technology Research Planning Project of Shaanxi Province (2012SF2-20-2)

摘要/Abstract

摘要： 目的: 研究点阵Er:YAG激光照射口腔黏膜后的组织学变化。方法: 使用Er:YAG激光照射家兔口腔黏膜,应用H-E、Masson、弹性纤维染色方法,评估照射后1、2、4周黏膜上皮厚度、固有层胶原纤维和弹性纤维含量。采用SPSS19.0软件包对实验结果进行统计学分析。结果: 点阵Er:YAG照射后,黏膜损伤区仅限黏膜上皮内,3~7 d后完全愈合,不遗留色素及瘢痕,黏膜上皮厚度显著增加（P<0.05）,固有层胶原纤维、弹性纤维含量显著增加（P<0.05）。点阵Er:YAG激光照射家兔口腔黏膜损伤小、愈合快,黏膜上皮重构,黏膜固有层胶原变性、收缩。结论: 点阵Er:YAG激光照射口腔黏膜后,胶原纤维及弹性纤维增生、重组,黏膜收紧,弹性增加。

关键词: Er:YAG激光, 胶原纤维, 弹性纤维, 兔

Abstract: PURPOSE: Histological changes were studied to evaluate the effect of fractional Er:YAG laser treatment on oral mucosa tissues. METHODS: The rabbit oral mucosa was irradiated with Er:YAG laser. The epithelial thickness, lamina propria collagen fiber and elastic fiber content were assessed at 1, 2, 4 weeks after OP. H-E staining, Masson staining and elastic fiber staining were utilized. The experimental results were statistically analyzed by SPSS19.0 software package. RESULTS: After dot matrix Er:YAG irradiation, mucosal damage zone was limited only within the mucosal epithelium, and healed completely in 3-7 days, without leaving scar and pigment. The epithelial thickness was increased significantly (P<0.05), and the lamina propria collagen fiber and elastic fiber content were increased significantly (P< 0.05). After fractional Er:YAG laser irradiation, the rabbit had a small oral mucosa lesion and healed quickly. The epithelial remodeled. The mucosa lamina propria collagen contracted. CONCLUSIONS: After fractional Er:YAG laser irradiation, collagen fiber and elastic fiber proliferated and remodeled. The mucosa was tightening and the mucous membrane elasticity was increased.

Key words: Er:YAG Laser, Collagen fiber, Elastic fiber, Rabbit

中图分类号:

R782.2

田海锁, 蔺栋鹏, 赵天一, 于擘, 马秦. 点阵Er:YAG激光照射兔口腔黏膜后的组织学变化[J]. 中国口腔颌面外科杂志, 2015, 13(2): 117-123.

TIAN Hai-suo, LIN Dong-peng, ZHAO Tian-yi, YU Bo, MA Qin. Histological change of rabbit oral mucosa after fractional Er:YAG laser irradiation[J]. China J Oral Maxillofac Surg, 2015, 13(2): 117-123.

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