p53上调凋亡调控因子在舌鳞癌中的表达及临床意义

中国口腔颌面外科杂志 ›› 2014, Vol. 12 ›› Issue (2): 121-125.

p53上调凋亡调控因子在舌鳞癌中的表达及临床意义

刘斌, 江方方, 余东升, 刘子锋, 赵玮

中山大学光华口腔医学院附属口腔医院口腔颌面外科,广东省口腔医学重点实验室,广东广州 510055

收稿日期:2013-07-18 修回日期:2013-09-05 出版日期:2014-03-10 发布日期:2014-11-06
通讯作者: 赵玮,Tel：020-83862543,E-mail：yuzhw992003@yahoo.com.cn
作者简介:刘斌(1979-),女 ,硕士,主治医师,E-mail: 13713389702@139.com,现在东莞市厚街医院口腔科工作
基金资助:
国家自然科学基金(30973340、81272554); 广东省自然科学基金(9151008901000187、S2011020003247); 广东省科技计划国际合作项目(2011B050400030、2012B031800387)

PUMA expression and its clinical significance in tongue squamous cell carcinoma

LIU Bin, JIANG Fang-fang, YU Dong-sheng, LIU Zi-feng, ZHAO Wei

Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Institute of Stomatological Research, Sun Yat-sen University. Guangzhou 510055, Guangdong Province, China

Received:2013-07-18 Revised:2013-09-05 Online:2014-03-10 Published:2014-11-06
Supported by:
National Natural Science Foundation of China (30973340 and 81272554); Guangdong Science and Technology Project (2011B050400030 and 2012B031800387); Natural Science Foundation of Guangdong Province (9151008901000187 and S2011020003247)

摘要/Abstract

摘要： 目的：观察p53上调凋亡调控因子(p53 up-regulated modulator of apoptosis,PUMA)在舌鳞癌组织、舌癌旁组织及正常舌黏膜组织中的表达,探讨其在舌鳞癌发生、发展中的作用及临床意义。方法：通过免疫组化检测30例舌鳞癌组织、13例舌癌旁组织及10例正常舌黏膜组织中PUMA的表达水平,分析PUMA在舌鳞癌中的表达与临床分期、肿瘤分化程度、淋巴结转移之间的关系。采用SPSS17.0软件包对数据进行统计学分析。结果：PUMA在舌鳞癌组织、舌癌旁组织及正常舌黏膜组织中的阳性表达率分别为36.67%、53.85%和80.00%,3组之间PUMA表达均有显著差异(P<0.05)。淋巴结转移组中PUMA的阳性表达率为18.18%,而无淋巴结转移组中PUMA的阳性表达率为47.37%,2组差异显著(P<0.05)。而在不同年龄、性别、临床与病理分期、肿瘤分化程度的舌鳞癌组织中,PUMA的表达差异无显著性(P>0.05)。结论：PUMA蛋白表达降低在舌鳞癌的发生、发展及转移过程中具有重要作用,对判断舌鳞癌的预后及治疗有一定的指导意义。

关键词: P53上调凋亡调控因子, 舌鳞癌, 免疫组织化学

Abstract: PURPOSE: To investigate the expression of PUMA (p53 up-regulated modulator of apoptosis) in tongue squamous cell carcinoma (TSCC), tongue tissues adjacent to cancer and normal tongue tissues, and to explore its clinical significance. METHODS: The expression of PUMA in resected samples from TSCC, tongue tissues adjacent to cancer and normal tongue tissues were detected by immunohistochemistry. The relationships between the expression of PUMA and the TNM staging, cell differentiation, and lymph node metastasis were analysed respectively. SPSS 17.0 software package was used for statistical analysis. RESULTS: Immunohistochemical studies demonstrated that PUMA expression was lower in TSCC (36.67%) than in tongue tissues adjacent to cancer(53.85%) and normal tongue tissues(80.00%). There was significant difference of PUMA expression in lymph node metastasis and lymph node without metastasis(P<0.05). The expression of PUMA in TSCC had no relation with age, gender, clinical stages, and the degree of cell differentiation(P>0.05). CONCLUSIONS: The results indicated that PUMA expression in TSCC is correlated with the pathogenesis and development of TSCC and lymph node metastasis and maybe a new promising target for treatment of TSCC.

Key words: p53 up-regulated modulator of apoptosis, Tongue squamous cell carcinoma, Immunohistochemistry

中图分类号:

R739.8

刘斌, 江方方, 余东升, 刘子锋, 赵玮. p53上调凋亡调控因子在舌鳞癌中的表达及临床意义[J]. 中国口腔颌面外科杂志, 2014, 12(2): 121-125.

LIU Bin, JIANG Fang-fang, YU Dong-sheng, LIU Zi-feng, ZHAO Wei. PUMA expression and its clinical significance in tongue squamous cell carcinoma[J]. China J Oral Maxillofac Surg, 2014, 12(2): 121-125.

参考文献

[1] Jemal A, Siegel R, Ward E, et al. Cancer statistics, 2009 [J]. CA Cancer J Clin, 2009, 59(4): 225-249.
[2] 严颖彬, 彭歆, 毛驰. 口腔癌患者术后生存质量评价及影响因素分析 [J]. 中国口腔颌面外科杂志, 2012, 10(4): 316-321.
[3] Yu J, Zhang L, Hwang PM, et al. PUMA induces the rapid apoptosis of colorectal cancer cells [J]. Mol Cell, 2001, 7(3): 673-682.
[4] Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53 [J]. Mol Cell, 2001, 7(3): 683-694.
[5] Kim MR, Jeong EG, Chae B, et al. Pro-apoptotic PUMA and anti-apoptotic phosphor-BAD are highly expressed in colorectal carcinomas [J]. Dig Dis Sci, 2007, 52(10): 2751-2756.
[6] Wang R, Wang X, Li B, et al. Tumor-specific adenovirus-mediated PUMA gene transfer using the survivin promoter enhances radiosensitivity of breast cancer cells in vitro and in vivo [J]. Breast Cancer Res Treat, 2009, 117(1): 45-54.
[7] Qiu W, Wang X, Leibowitz B, et al. PUMA-mediated apoptosis drives chemical hepatocarcinogenesis in mice [J]. Hepatology, 2011, 54(4): 1249-1258.
[8] 江方方, 余东升. p53上调凋亡调控因子在肿瘤中的作用及其在口腔癌研究中的新进展[J]. 广东牙病防治, 2012, 20(9): 498-501.
[9] Max J. Cancer research. Anti-inflammatories inhibit cancer growth-but how? [J]. Science, 2001, 291(5504): 581-582.
[10] Karst AM, Dai DL, Martinka M, et al. PUMA expression is significantly reduced in human cutaneous melanomas [J]. Oncogene, 2005, 4(6): 1111-1116.
[11] 张克君, 李德春, 朱东明. PUMA 蛋白在胰腺癌中的表达及临床意义 [J]. 世界华人消化杂志, 2008, 16(5): 488-492.
[12] Jansson A, Arbman C, Sun XF, et al. mRNA and protein expression of PUMA in sporadic colorectal cancer [J]. Oncol Rep, 2004, 12(6): 1245-1249.
[13] Yoo NJ, Lee JW, Jeonq EG, et al. Immunohistochemical analysis of pro-apoptotic PUMA protein and mutational analysis of PUMA gene in gastric carcinoma[J]. Dig Liver Dis, 2007, 39(3):222-227.
[14] Ahn CH, Jeong EG, Kim SS, et al. Expressional and mutational analysis of pro-apoptotic Bcl-2 member PUMA in hepatocellular carcinomas [J]. Dig Dis Sci, 2008, 53(5): 1395-1399.
[15] Coutinho-Camillo CM, Lourenço SV, Nishimoto IN, et al. Expression of Bcl-2 family proteins and association with clinicopathological characteristics of oral squamous cell carcinoma [J]. Histopathology, 2010, 57(2): 304-316.