正畸牙移入对牙槽突裂植骨区骨改建的生物学影响

中国口腔颌面外科杂志 ›› 2014, Vol. 12 ›› Issue (2): 120-124.

正畸牙移入对牙槽突裂植骨区骨改建的生物学影响

孙健, 陈振琦

上海交通大学医学院附属第九人民医院·口腔医学院口腔正畸科,上海市口腔医学重点实验室,上海 200011

收稿日期:2013-10-14 修回日期:2013-11-18 出版日期:2014-03-10 发布日期:2014-11-06
通讯作者: 陈振琦,E-mail：orthochen@yeah.net
作者简介:孙健(1989-),女,在读硕士研究生,E-mail：sunjian198903@163.com
基金资助:
上海市科学技术委员会科研计划项目(124119b0103)

The biological effect of orthodontic tooth moving into alveolar cleft bone grafting area

SUN Jian, CHEN Zhen-qi

Department of Orthodontics, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2013-10-14 Revised:2013-11-18 Online:2014-03-10 Published:2014-11-06
Supported by:
Scientific Research Projects of Science and Technology Committee of Shanghai Municipality (124119b0103)

摘要/Abstract

摘要： 目的：通过检测正畸牙移入后牙槽突裂植骨区内Trap、Rankl、Runx2的表达,研究正畸牙移入对牙槽突裂植骨区骨改建的生物学影响。方法：选用12只8周龄SD雄性大鼠,制备正畸牙移入牙槽突裂植骨区的动物模型,加力前及加力1、3、5 d后处死动物取材,进行H-E染色,检测裂隙区骨形成情况,采用实时荧光定量PCR检测Trap、Rankl、Runx2表达,应用SPSS 13.0软件包对数据进行两样本均数t检验。结果：Trap在早、中期升高,Rankl在早期升高,Runx2在晚期升高。结论：正畸牙移入加强了植入骨的骨重建,诱导了破骨和以后的成骨过程。

关键词: 正畸牙, 牙槽突裂, 植骨区, 实时荧光定量PCR, 骨改建

Abstract: PURPOSE: To study the biological effects of orthodontic tooth movement into alveolar cleft bone graft area, and evaluate expression of Trap/Rankl/Runx2 by real-time PCR. METHODS: The model of orthodontic tooth movement into alveolar cleft bone graft area was established in 8-week-old SD rats. The animals were sacrificed before orthodontic treatment and 1, 3, 5 days after orthodontic treatment. Bone formation was evaluated by H-E staining and the expression of Trap/Rankl/Runx2 was detected by Real-time PCR. SPSS 13.0 software package was used for two sample paired t test. RESULTS: The expression of Trap increased at early and middle stage, Rankl increased at early stage and Runx2 increased at late stage. CONCLUSIONS: Orthodontic tooth movement into alveolar cleft bone graft area promoted bone remodeling of embedded bone, induced osteoclastic process and subsequent osteogenesis.

Key words: Orthodontic tooth, Alveolar cleft, Bone graft area, Real-time PCR, Bone remodel

中图分类号:

R783.5

孙健, 陈振琦. 正畸牙移入对牙槽突裂植骨区骨改建的生物学影响[J]. 中国口腔颌面外科杂志, 2014, 12(2): 120-124.

SUN Jian, CHEN Zhen-qi. The biological effect of orthodontic tooth moving into alveolar cleft bone grafting area[J]. China J Oral Maxillofac Surg, 2014, 12(2): 120-124.

参考文献

[1] Hossain Mz, Kyomen S, Tanne K. Biologic responses of autogenous bone and beta-tricalcium phosphate ceramics transplanted into bone defects to orthodontic forces [J]. Cleft Palate Craniofac J, 1996, 33(4): 277-283.
[2] Liao HT, Chen CH, Bergeron L, et al. Alveolar bone grafting in the treatment of midline alveolar cleft and diastema in incomplete median cleft lip [J]. Int J Oral Maxillofac Surg, 2008, 37(10): 886-891.
[3] Kawamoto T, Motohashi N, Kitamura A, et al. Experimental tooth movement into bone induced by recombinant human bone morphogenetic protein-2[J]. Cleft Palate Craniofac J, 2003, 40(5): 538-543.
[4] Tanimoto K, Sumi K, Yoshioka M, et al. Experimental tooth movement into new bone area regenerated by use of bone marrow-derived mesenchymal stem cells [J]. Cleft Palate Craniofac J, 2013 Jun 19. [Epub ahead of print]
[5] Uji M. Histological study on experimental tooth movement in alveolar bone defects implanted with decalcified bone powder [J]. Kokubyo Gakkai Zasshi, 1996, 63(1): 208-221.
[6] Seifi M, Ghoraishian SA. Determination of orthodontic tooth movement and tissue reaction following demineralized freeze-dried bone allograft grafting intervention [J]. Dent Res J (Isfahan), 2012, 9(2): 203-208.
[7] Ren Y, Vissink A. Cytokines in crevicular fluid and orthodontic tooth movement [J]. Eur J Oral Sci, 2008, 116(2): 89-97.
[8] 许悦, 陈振琦, 吴军, 等. 唇腭裂合并的单侧牙槽突裂大鼠模型的建立和稳定性研究 [J]. 上海交通大学学报(医学版), 2010, 30(9): 1111-1114.
[9] Kawamoto T, Motohashi N, Kitamura A, et al. A histological study on experimental tooth movement into bone induced by recombinant human bone morphogenetic protein-2 in beagle dogs [J]. Cleft Palate Craniofac J, 2002, 39(4): 439-448.
[10] 李壬媚, 许悦, 陈振琦, 等. 不同时机牙移动对SD大鼠牙槽突裂植骨区骨改建的影响[J].中国口腔颌面外科杂志,2012,10(2): 96-100.
[11] Fujita S, Yamaguchi M, Utsunomiya T, et al. Low-energy laser stimulates tooth movement velocity via expression of RANK and RANKL [J]. Orthod Craniofac Res, 2008, 11(3): 143-155.
[12] Mason DJ, Hillam RA, Skerry TM. Constitutive in vivo mRNA expression by osteocytes of beta-actin, osteocalcin, connexin-43, IGF-I, c-fos and c-jun, but not TNF-alpha nor tartrate-resistant acid phosphatase [J]. J Bone Miner Res, 1996, 11(3): 350-357.
[13] Kiechl S, Werner P, Knoflach M, et al. The osteoproteger in/RANK/RANKL system: a bone key to vascular disease [J]. Expert Rev Cardiovasc Ther, 2006, 4(6): 801-811.
[14] Fuller K, Wong B, Fox S, et al. TRANCE is necessary and sufficient for osteoblast-mediated activation of bone resorption in osteoclasts [J]. J Exp Med, 1998, 188(5): 997-1001.
[15] Bronckers Al, Sasaguri K, Engelse MA. Transcription and immunolocalization of Runx2/Cbfa1/Pebp2alphaA in developing rodent and human craniofacial tissues: further evidence suggesting osteoclasts phagocytose osteocytes [J]. Microsc Res Tech, 2003, 61(6): 540-548.