Rap1B在舌鳞状细胞癌中的表达及对细胞增殖、侵袭和迁移的影响

doi:10.19438/j.cjoms.2026.03.004

中国口腔颌面外科杂志 ›› 2026, Vol. 24 ›› Issue (3): 223-233.doi: 10.19438/j.cjoms.2026.03.004

Rap1B在舌鳞状细胞癌中的表达及对细胞增殖、侵袭和迁移的影响

徐勇¹, 崔倩², 邵禹³, 徐进¹, 于汶源⁴, 陈正岗^1,4

1.山东医药大学口腔医学院,山东烟台 264003;
2.康复大学青岛医院（青岛市市立医院）眼科口腔科,山东青岛 266001;
3.山东第二医科大学口腔医学院,山东潍坊 261053;
4.山东医药大学口腔医院,山东烟台 264003

收稿日期:2025-08-01 修回日期:2025-10-17 出版日期:2026-05-20 发布日期:2026-06-04
通讯作者: 陈正岗,E-mail：chenzhg1973@163.com
作者简介:徐勇（1998—）,男,硕士,E-mail：1790197281@qq.com
基金资助:
山东省医药卫生科技项目（202308020420）

The Expression of Rap1B in tongue squamous cell carcinoma and its effects on cell proliferation, invasion and migration

Xu Yong¹, Cui Qian², Shao Yu³, Xu Jin¹, Yu Wenyuan⁴, Chen Zhenggang^1,4

1. College of Stomatology, Shandong Medical and Pharmaceutical University. Yantai 264003;
2. Department of Ophthalmology and Stomatology, Qingdao Hospital, University of Health and Rehabilitation Sciences (Qingdao Municipal Hospital). Qingdao 266001;
3. School of Stomatology, Shandong Second Medical University. Weifang 261053;
4. Affiliated Stomatological Hospital, Shandong Medical and Pharmaceutical University. Yantai 264003, Shandong Province, China

Received:2025-08-01 Revised:2025-10-17 Online:2026-05-20 Published:2026-06-04

摘要/Abstract

摘要： 目的: 探讨Ras相关蛋白1B（Rap1B）在舌鳞状细胞癌（TSCC）中的表达及其与患者临床病理参数和预后的相关性,并通过体外实验研究Rap1B基因沉默对TSCC细胞增殖、凋亡、侵袭、迁移和上皮-间充质转化（EMT）的影响及其潜在分子机制。方法: 基于GEPIA数据库,分析Rap1B在TSCC组织中的表达。通过蛋白免疫印迹（Western blot）和免疫组织化学染色（IHC）检测组织中Rap1B蛋白表达水平,分析其表达与患者临床病理特征及预后的相关性。用siRNA沉默Rap1B后,采用Western blot检测Rap1B、PI3K、Akt、磷酸化Akt、mTOR、磷酸化mTOR、p70S6K、磷酸化p70S6K以及EMT相关标志物E-cadherin、N-cadherin、Vimentin的表达。通过CCK-8实验、EdU实验、流式细胞术、Transwell侵袭实验、伤口愈合实验评价细胞活性、增殖、凋亡、侵袭和迁移能力的改变。结果: Rap1B在TSCC中高表达（P<0.05）。Rap1B表达水平与TSCC患者的临床TNM分期、肿瘤实质浸润深度以及颈淋巴结转移状态呈显著相关性（P<0.05）。Rap1B基因沉默后,实验组细胞中PI3K、p-AKT、p-mTOR、p-p70S6K及N-cadherin、Vimentin的蛋白表达显著下调（P<0.05）,而E-cadherin表达显著升高（P<0.05）。Rap1B沉默导致TSCC细胞活性、增殖、侵袭和迁移能力显著下降（P<0.05）,细胞凋亡率显著升高（P<0.05）。结论: Rap1B在TSCC中高表达,其通过调控PI3K/AKT/mTOR/p70S6K信号通路的磷酸化水平,促进肿瘤细胞增殖、侵袭、迁移及EMT进程,并抑制细胞凋亡。靶向沉默Rap1B,可显著抑制上述恶性生物学行为,提示Rap1B有望成为TSCC精准治疗的潜在分子靶点。

关键词: 舌鳞状细胞癌, Rap1B, 增殖, 凋亡, 上皮-间充质转化

Abstract: PURPOSE: To investigate the expression of Ras-related protein 1B (Rap1B) in tongue squamous cell carcinoma (TSCC) and its correlation with clinicopathological parameters and patient prognosis, while examining the effects of Rap1B silencing on TSCC cell proliferation, apoptosis, invasion, migration, and epithelial-mesenchymal transition (EMT) through in vitro experiments, along with exploring the underlying molecular mechanisms. METHODS: Rap1B expression in TSCC tissues was initially assessed using the GEPIA database. Rap1B protein levels in tissues were determined by Western blot and immunohistochemistry (IHC). The association between Rap1B expression and clinicopathological features, as well as patients' prognosis, was statistically evaluated. Following silencing of Rap1B by small interfering RNA (siRNA), Western blotting was employed to quantify the expression levels of Rap1B, PI3K, Akt, phosphorylated Akt (p-Akt), mTOR, phosphorylated mTOR (p-mTOR), p70S6K, phosphorylated p70S6K (p-p70S6K), and EMT-related markers (E-cadherin, N-cadherin, Vimentin). Cellular viability, proliferation, apoptosis, invasion, and migration were evaluated through CCK-8 assay, EdU assay, flow cytometry, Transwell invasion assay, and wound healing assay, respectively. RESULTS: Rap1B exhibited high expression in TSCC tissues (P<0.05). Rap1B expression levels demonstrated significant correlations with clinical TNM stage, depth of tumor invasion, and cervical lymph node metastasis status in TSCC (P<0.05). After Rap1B silencing, the protein expression levels of PI3K, p-Akt, p-mTOR, p-p70S6K, N-cadherin, and Vimentin in the experimental group were markedly downregulated (P<0.05), whereas the expression of E-cadherin was significantly upregulated (P<0.05). Silencing of Rap1B resulted in a significant reduction in TSCC cellular viability, proliferation, invasion, and migration (P<0.05), concomitant with a significant elevation in the apoptosis rate (P<0.05). CONCLUSIONS: Rap1B is highly expressed in TSCC. It facilitates tumor cell proliferation, invasion, migration, and EMT, while inhibiting apoptosis, through modulation of the phosphorylation status of the PI3K/AKT/mTOR/p70S6K signaling pathway. Targeted silencing of Rap1B can significantly suppress these malignant biological behaviors, indicating that Rap1B holds potential as a candidate molecular target for precision therapy in TSCC.

Key words: Tongue squamous cell carcinoma, Rap1B, Proliferation, Invasion, Epithelial-mesenchymal transition

中图分类号:

R739.86

徐勇, 崔倩, 邵禹, 徐进, 于汶源, 陈正岗. Rap1B在舌鳞状细胞癌中的表达及对细胞增殖、侵袭和迁移的影响[J]. 中国口腔颌面外科杂志, 2026, 24(3): 223-233.

Xu Yong, Cui Qian, Shao Yu, Xu Jin, Yu Wenyuan, Chen Zhenggang. The Expression of Rap1B in tongue squamous cell carcinoma and its effects on cell proliferation, invasion and migration[J]. China J Oral Maxillofac Surg, 2026, 24(3): 223-233.

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Rap1B在舌鳞状细胞癌中的表达及对细胞增殖、侵袭和迁移的影响

The Expression of Rap1B in tongue squamous cell carcinoma and its effects on cell proliferation, invasion and migration

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