HER2基因在唾液腺恶性多形性腺瘤细胞增殖、周期、凋亡中的作用

doi:10.19438/j.cjoms.2018.04.004

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (4): 310-316.doi: 10.19438/j.cjoms.2018.04.004

HER2基因在唾液腺恶性多形性腺瘤细胞增殖、周期、凋亡中的作用

夏亮^1,2,*, 汪洋^1,*, 胡宇华¹, 王丽珍¹, 顾挺¹, 李江¹, 田臻¹

1.上海交通大学医学院附属第九人民医院·口腔医学院口腔病理科,
2.口腔颅颌面科,国家口腔疾病临床研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011

收稿日期:2017-10-16 修回日期:2017-12-25 出版日期:2018-07-20 发布日期:2018-08-09
通讯作者: 田臻,E-mail：tian0304_cn@163.com
作者简介:夏亮（1990-）,男,E-mail：anglexialiang@126.com;汪洋（1989-）,男,E-mail：cmfuyangwang@126.com。
基金资助:
国家自然科学基金（81272976,81372910）

Effect of HER2 gene on cell proliferation, cell cycle and apoptosis in salivary malignant pleomorphic adenoma

XIA Liang^1,2, WANG Yang¹, HU Yu-hua¹, WANG Li-zhen¹, GU Ting¹, LI Jiang¹, TIAN Zhen¹

1.Department of Oral Pathology,
2.Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology. Shanghai 200011, China;

Received:2017-10-16 Revised:2017-12-25 Online:2018-07-20 Published:2018-08-09

摘要/Abstract

摘要： 目的:通过体外抑制唾液腺恶性多形性腺瘤（malignant pleomorphic adenoma,MPA）中人表皮生长因子受体2（Human epithelial growth factor receptor 2,HER2）,分析其与肿瘤细胞增殖、周期和凋亡等恶性生物学行为的相关性,以期评估其作为靶向治疗位点的可能性。方法:检测MPA细胞系中HER2蛋白表达及基因扩增情况,应用靶向抑制剂处理肿瘤细胞,检测肿瘤增殖、周期、凋亡等生物学行为的改变,HER2蛋白表达改变及其对下游通路的影响。采用SPSS 16.0软件包进行独立样本t检验。结果:SM-AP1、SM-AP4细胞中均存在HER2蛋白表达及HER2基因扩增,应用HER2靶向抑制剂可以显著降低肿瘤细胞的增殖能力,阻滞细胞周期进程,诱导肿瘤细胞凋亡,并且HER2下游PI3K/Akt和MAPK/ERK细胞通路受到抑制。结论:MPA肿瘤细胞系中存在HER2基因过表达及扩增,HER2在MPA恶性生物学行为中扮演重要角色。抑制HER2,可以改善MPA的恶性生物学行为。

关键词: HER2, 恶性多形性腺瘤, 唾液腺

Abstract: PURPOSE:To inhibit HER2 in salivary malignant pleomorphic adenoma, investigate the effect on tumor cell proliferation, cell cycle and apoptosis, and assess the possibility of blocking HER2 to improve MPA malignant biological behavior. METHODS: HER2 expression and proliferation were detected in MPA cell lines. The effect on tumor cell proliferation, cell cycle, apoptosis and down-stream pathway were verified after use of HER2 blockade. SPSS 16.0 software package was used for statistical analysis. RESULTS: HER2 overexpressed/proliferated in SM-AP1 and SM-AP4 cells. By blocking HER2, tumor proliferation and cell cycle were significantly induced, and apoptosis process was activated. In addition, the downstream pathway PI3K/Akt and MAPK/ERK were significantly inhibited. CONCLUSIONS: HER2 overexpressed/proliferated in MPA cell line, which might play an important role in tumor genesis. Inhibiting HER2 might be a novel therapy for poor biological behavior on MPA.

Key words: HER2, Malignant pleomorphic tumor, Salivary gland

中图分类号:

R739.8

夏亮, 汪洋, 胡宇华, 王丽珍, 顾挺, 李江, 田臻. HER2基因在唾液腺恶性多形性腺瘤细胞增殖、周期、凋亡中的作用[J]. 中国口腔颌面外科杂志, 2018, 16(4): 310-316.

XIA Liang, WANG Yang, HU Yu-hua, WANG Li-zhen, GU Ting, LI Jiang, TIAN Zhen. Effect of HER2 gene on cell proliferation, cell cycle and apoptosis in salivary malignant pleomorphic adenoma[J]. China J Oral Maxillofac Surg, 2018, 16(4): 310-316.

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HER2基因在唾液腺恶性多形性腺瘤细胞增殖、周期、凋亡中的作用

Effect of HER2 gene on cell proliferation, cell cycle and apoptosis in salivary malignant pleomorphic adenoma

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