抗菌肽对TNF-α诱导成骨细胞凋亡的抑制作用

doi:10.19438/j.cjoms.2025.04.003

中国口腔颌面外科杂志 ›› 2025, Vol. 23 ›› Issue (4): 325-330.doi: 10.19438/j.cjoms.2025.04.003

抗菌肽对TNF-α诱导成骨细胞凋亡的抑制作用

谢丽敏¹, 刘颖², 王茹¹, 刘丹丹³, 李佑坤⁴, 周东升¹, 杨德圣⁵, 李喜峰¹

1.菏泽市立医院口腔科,2.药学部,3.消化内科,山东菏泽 274000;
4.鄄城马爱云医院口腔科,山东菏泽 274699;
5.北京大学首钢医院口腔科,北京 100144

收稿日期:2025-03-12 修回日期:2025-05-06 出版日期:2025-07-20 发布日期:2025-08-04
通讯作者: 李喜峰,E-mail：lixifeng88@126.com
作者简介:谢丽敏（1987-）,女,硕士研究生,主治医师,E-mail：690735790@qq.com

The inhibitory effect of antibacterial peptides on osteoblast apoptosis induced by TNF-α

Xie Limin¹, Liu Ying², Wang Ru¹, Liu Dandan³, Li Youkun⁴, Zhou Dongsheng¹, Yang Desheng⁵, Li Xifeng¹

1. Department of Stomatology, 2. Department of Pharmacy, 3. Department of Gastroenterology, Heze Municipal Hospital. Heze 274000, Shandong Province;
4. Department of Stomatology, Juancheng Maaiyun Hospital. Heze 274699, Shandong Province;
5. Department of Stomatology, Peking University Shougang Hospital. Beijing 100144, China

Received:2025-03-12 Revised:2025-05-06 Online:2025-07-20 Published:2025-08-04

摘要/Abstract

摘要： 目的：探讨抗菌肽对肿瘤坏死因子α（TNF-α）诱导的成骨细胞凋亡的抑制作用。方法：将成骨细胞分为MEM对照组、抗菌肽组、TNF-α组和TNF-α+抗菌肽组。成骨细胞培养 1 d 后,抗菌肽组加入终末浓度为20 μg/mL 的抗菌肽,TNF-α组、TNF-α+抗菌肽组加入 50 ng/mL TNF-α诱导培养1 d 后,TNF-α+抗菌肽组加入终末浓度 20 μg/mL 的抗菌肽继续培养1 d。流式细胞仪检测细胞凋亡情况。培养1、2、3、4 d后,划痕法检测细胞迁移能力。培养4 d后,检测细胞碱性磷酸酶活性。培养21 d后,茜素红染色法检测细胞成骨能力。细胞连续传代培养3次,显微镜下观察成骨细胞形态,流式细胞仪检测细胞凋亡情况。结果：TNF-α组成骨细胞的形状不规则,细胞凋亡增多,细胞迁移慢,钙结节量少,碱性磷酸酶活性降低,细胞传代过程中凋亡增多、形状改变。MEM对照组、抗菌肽组和TNF-α+抗菌肽组成骨细胞形态、凋亡、迁移、钙结节、碱性磷酸酶活性及细胞传代均无显著改变。结论：TNF-α能够诱导成骨细胞凋亡,而抗菌肽能够抑制TNF-α诱导的成骨细胞凋亡。

关键词: 成骨细胞, 抗菌肽, TNF-α, 凋亡

Abstract: PURPOSE: To explore the inhibitory effect of antimicrobial peptides on osteoblast apoptosis induced by tumor necrosis factor-α(TNF-α). METHODS: Osteoblasts were divided into MEM control group, antimicrobial peptide group, TNF-α group, and TNF-α+ antimicrobial peptide group. After 1 day of osteoblast culture, the antimicrobial peptide group was added with a final concentration of 20 μg/mL of antimicrobial peptide. The TNF-α group and the TNF-α + antimicrobial peptide group were induced with 50 ng/mL TNF-α for 1 day. Then, the TNF-α + antimicrobial peptide group was added with a final concentration of 20 μg/mL of antimicrobial peptide and continued to be cultured for 1 day. Apoptosis of cells was detected by flow cytometry. Cell migration ability was detected by scratch assay at 1, 2, 3 and 4 days of culture. Alkaline phosphatase activity was detected at 4 days of culture. Osteogenic ability was detected by alizarin red staining at 21 days of culture. Cells were continuously passaged three times, and the morphology of osteoblasts was observed under microscope. Cell apoptosis was detected by flow cytometry. RESULTS: In TNF-α group, the shape of osteocytes was irregular, the number of apoptotic cells increased, the migration of osteocytes was slow, the amount of calcium nodules decreased, the activity of alkaline phosphatase decreased, the apoptosis of osteocytes increased and the shape changed during cell passage. The MEM control group, the antimicrobial peptide group and the TNF-α+ antimicrobial peptide group had no change on the morphology, apoptosis, migration, calcium nodules, alkaline phosphatase activity and cell passage of osteoblasts. CONCLUSIONS: Osteoblast apoptosis can be induced by TNF-α, and the osteoblast apoptosis induced by TNF-α can be inhibited by antibacterial peptide.

Key words: Osteoblast, Antibacterial peptide, TNF-α, Apoptosis

中图分类号:

Q28
R782

谢丽敏, 刘颖, 王茹, 刘丹丹, 李佑坤, 周东升, 杨德圣, 李喜峰. 抗菌肽对TNF-α诱导成骨细胞凋亡的抑制作用[J]. 中国口腔颌面外科杂志, 2025, 23(4): 325-330.

Xie Limin, Liu Ying, Wang Ru, Liu Dandan, Li Youkun, Zhou Dongsheng, Yang Desheng, Li Xifeng. The inhibitory effect of antibacterial peptides on osteoblast apoptosis induced by TNF-α[J]. China J Oral Maxillofac Surg, 2025, 23(4): 325-330.

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抗菌肽对TNF-α诱导成骨细胞凋亡的抑制作用

The inhibitory effect of antibacterial peptides on osteoblast apoptosis induced by TNF-α

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