细胞凋亡及上皮-间充质转化在腭胚突融合过程中的作用探讨

doi:10.19438/j.cjoms.2018.06.003

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (6): 493-497.doi: 10.19438/j.cjoms.2018.06.003

细胞凋亡及上皮-间充质转化在腭胚突融合过程中的作用探讨

马利^1,2, 石冰², 张东升¹, 郑谦²

1.山东大学附属省立医院口腔科,山东济南 250021;
2.四川大学华西口腔医院唇腭裂外科,四川成都 610041

收稿日期:2018-03-08 出版日期:2018-11-20 发布日期:2019-01-11
通讯作者: 郑谦,E-mail：zq652@163.com
作者简介:马利（1983-）,男,博士研究生,主治医师,E-mail:slyykqhmwk@163.com

The effect of apoptosis and epithelial-mesenchymal transition during palatal fusion

MA Li^1,2, SHI Bing², ZHANG Dong-sheng¹, ZHENG Qian²

1.Department of Stomatology, Shandong Provincial Hospital affiliated to Shandong University. Jinan 250021, Shandong Province;
2.Department of Cleft Lip and Palate Surgery, West China Stomatological Hospital, Sichuan University. Chengdu 610041, Sichuan Province, China

Received:2018-03-08 Online:2018-11-20 Published:2019-01-11

摘要/Abstract

摘要： 目的: 探讨细胞凋亡及上皮-间充质转化（epithelial-mesenchymal transition, EMT）在腭胚突融合中的作用。方法: 选用8周龄C57BL/6J近交系小鼠作为研究对象。于E13.5、E14.0、E14.5、E15.5及E17.5 d获取胎鼠腭胚突组织,在腭胚突融合的关键阶段,应用免疫组织化学和TUNEL方法检测EMT过程中蛋白变化和细胞凋亡率。每个时间点选择3张图片,应用image J软件进行图片灰度分析,采用SPSS12.0软件包进行统计学分析。结果: 免疫组织化学和TUNEL法检测发现在腭融合的关键阶段（E14.5）,腭中嵴上皮（medial edge epithelium, MEE）中出现纤连蛋白和波形蛋白表达和细胞凋亡,前中后腭部MEE细胞也出现细胞凋亡,中后部细胞凋亡率显著高于前部。结论: 在体内腭胚突融合的关键阶段,EMT过程和细胞凋亡均参与了腭中嵴上皮带的退化消失,且前中后腭部的融合机制基本一致。

关键词: 腭裂, 腭胚突, 细胞凋亡, 上皮-间充质转化

Abstract: PURPOSE: Disappearance mechanism of the medial edge epithelium (MES) in the process of palatal fusion is still unknown. The aim of this study was to investigate the effect of apoptosis and epithelial-mesenchymal transition (EMT) during palatal fusion. METHODS: 8-week-old C57BL/6J inbred mice were selected as the experimental mouse. The palatal shelves were acquired at E13.5, E14.0, E14.5, E15.5 and E17.5. Immunohistochemistry and TUNEL were used to explore the protein expression and cell apoptosis during the key process of palatal fusion. Three pictures were randomly selected at each time point, and gray images were analyzed by Image J software. SPSS12.0 software package was used for statistical analysis. RESULTS: Immunohistochemistry and TUNEL assay found that the expressions of fibronectin and vimentin in MES and apoptosis were detected during the critical stage of palate fusion (E14.5), and the two processes were detected in the anterior, middle and posterior palate shelves. CONCLUSIONS: EMT and apoptosis were involved in the degeneration of MES, and the fusion mechanism of the anterior palate was the same as the posterior palate.

Key words: Cleft palate, Palatal shelves, Apoptosis, Epithelial-mesenchymal transition

中图分类号:

R782.2

马利, 石冰, 张东升, 郑谦. 细胞凋亡及上皮-间充质转化在腭胚突融合过程中的作用探讨[J]. 中国口腔颌面外科杂志, 2018, 16(6): 493-497.

MA Li, SHI Bing, ZHANG Dong-sheng, ZHENG Qian. The effect of apoptosis and epithelial-mesenchymal transition during palatal fusion[J]. China J Oral Maxillofac Surg, 2018, 16(6): 493-497.

参考文献

[1] Ma L, Shi B, Zheng Q.Targeted mutations of genes reveal important roles in palatal development in mice[J]. Ann Plast Surg, 2015, 74(2): 263-268.
[2] Dudas M, Li WY, Kim J, et al.Palatal fusion-Where do the midline cells go? A review on cleft palate, a major human birth defect[J]. Acta Histochem, 2007, 109(1): 1-14.
[3] Iwata J, Suzuki A, Yokota T, et al.TGFβ regulates epithelial-mesenchymal interactions through WNT signaling activity to control muscle development in the soft palate[J]. Development, 2014, 141(4): 909-917.
[4] Nawshad A, LaGamba D, Hay ED. Transforming growth factor β (TGFβ) signalling in palatal growth, apoptosis and epithelial mesenchymal transformation (EMT)[J]. Arch Oral Biol, 2004, 49(9): 675-689.
[5] Shuler CF, Halpern DE, Guo Y, et al.Medial edge epithelium fate traced by cell lineage analysis during epithelial-mesenchymal transformation in vivo[J]. Dev Biol, 1992, 154(2): 318-330.
[6] Carette MJ, Ferguson MW.The fate of medial edge epithelial cells during palatal fusion in vitro: an analysis by DiI labelling and confocal microscopy[J]. Development, 1992, 114(2): 379-388.
[7] Sun D, Vanderburg CR, Odierna GS, et al.TGF-beta3 promotes transformation of chicken palate medial edge epithelium to mesenchyme in vitro[J]. Development, 1998, 125(1): 95-105.
[8] Iwata J, Suzuki A, Pelikan RC, et al.Smad4-Irf6 genetic interaction and TGFβ-mediated IRF6 signaling cascade are crucial for palatal fusion in mice[J]. Development, 2013, 140(6): 1220-1230.
[9] Nelson ER, Levi B, Longaker MT.Commentary on role of apoptosis in retinoic Acid-induced cleft palate[J]. J Craniofac Surg, 2011, 22(5): 1572-1573.
[10] Vaziri Sani F, Hallberg K, Harfe BD, et al.Fate-mapping of the epithelial seam during palatal fusion rules out epithelial-mesenchymal transformation[J]. Dev Biol, 2005, 285(2): 490-495.
[11] Takahara S, Takigawa T, Shiota K.Programmed cell death is not a necessary prerequisite for fusion of the fetal mouse palate[J]. Int J Dev Biol, 2004, 48(1): 39-46.
[12] Lan Y, Xu J, Jiang R.Cellular and molecular mechanisms of palatogenesis[J]. Curr Top Dev Biol, 2015, 115: 59-84.
[13] Nieto MA, Huang RY, Jackson RA, et al.EMT: 2016[J]. Cell, 2016, 166(1): 21-45.
[14] Diepenbruck M, Christofori G.Epithelial-mesenchymal transition (EMT) and metastasis: yes, no, maybe?[J]. Curr Opin Cell Biol, 2016, 43: 7-13.
[15] LaGamba D, Nawshad A, Hay ED. Microarray analysis of gene expression during epithelial-mesenchymal transformation[J]. Dev Dyn, 2005, 234(1): 132-142.
[16] Ke CY, Xiao WL, Chen CM, et al.IRF6 is the mediator of TGFβ3 during regulation of the epithelial mesenchymal transition and palatal fusion[J]. Sci Rep, 2015, 5: 12791.
[17] Riedl SJ, Shi Y.Molecular mechanisms of caspase regulation during apoptosis[J]. Nat Rev Mol Cell Biol, 2004, 5(11): 897-907.
[18] Taniguchi K, Sato N, Uchiyama Y.Apoptosis and heterophagy of medial edge epithelial cells of the secondary palatine shelves during fusion[J]. Arch Histol Cytol, 1995, 58(2): 191-203.

细胞凋亡及上皮-间充质转化在腭胚突融合过程中的作用探讨

The effect of apoptosis and epithelial-mesenchymal transition during palatal fusion

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	周鑫霞，刘敬浩，甘桂芳，陈福祥. 鞘氨醇-1-磷酸受体4在口腔鳞状细胞癌中的表达及生物学功能[J]. 中国口腔颌面外科杂志, 2024, 22(1): 10-15.
[2]	高涛, 马莲, 罗奕, 孙勇刚. 鼻音计鼻音化率值与腭咽闭合状态关系的探讨[J]. 中国口腔颌面外科杂志, 2023, 21(4): 372-376.
[3]	蓝春慧, 张雪燕, 韦敏玲, 丁文炜, 梁建华. 整体性护理路径在先天性腭裂患儿手术护理中的应用[J]. 中国口腔颌面外科杂志, 2023, 21(3): 237-242.
[4]	褚珺, 胡明, 王国民, 吕挺正. 19例合并凝血功能异常的腭裂患儿围术期处理[J]. 中国口腔颌面外科杂志, 2022, 20(3): 282-286.
[5]	陈正岗, 王奇民, 童磊, 王云英, 徐晓娜, 王莹, 韩红钰, 盛善桂, 王少如. 沉默Icmt基因对舌鳞癌CAL-27和SCC-4细胞增殖、凋亡及细胞周期的影响[J]. 中国口腔颌面外科杂志, 2021, 19(2): 97-104.
[6]	张凌, 仇琳, 纪均. 喷他佐辛预防儿童腭裂手术全麻苏醒期躁动的效果评价[J]. 中国口腔颌面外科杂志, 2021, 19(1): 40-44.
[7]	林晓锐, 李晨曦, 张秀华. 瑞芬太尼复合氯胺酮在婴幼儿唇腭裂手术中的麻醉效果分析[J]. 中国口腔颌面外科杂志, 2021, 19(1): 45-48.
[8]	赵欣然, 蔡鸣. 不同咽成形术式对腭裂术后继发腭咽闭合功能不全合并睡眠呼吸障碍的影响[J]. 中国口腔颌面外科杂志, 2021, 19(1): 76-81.
[9]	徐桥石, 杨溪, 马海龙, 常涵月, 张陈平. 白细胞介素8在口腔鳞癌上皮-间充质转化中的研究进展[J]. 中国口腔颌面外科杂志, 2020, 18(6): 563-566.
[10]	牟思璇, 朱妍菲, 杜常欣, 王雪纯, 吴艳棋, 梅鹏, 王旭东, 朱敏. 改良牙支持式上颌骨前部牵引成骨术与上颌骨Le Fort I型前徙术对唇腭裂患者腭咽部结构的影响[J]. 中国口腔颌面外科杂志, 2020, 18(5): 427-433.
[11]	张晓敏, 杨雅娴, 宗源, 林鸣. Noordhoff手术联合PNAM正畸治疗唇腭裂患儿的疗效评价[J]. 中国口腔颌面外科杂志, 2020, 18(4): 352-356.
[12]	张文静, 王霞. 非综合征唇裂伴或不伴腭裂对口腔健康相关生活质量的影响[J]. 中国口腔颌面外科杂志, 2020, 18(3): 255-259.
[13]	刘国新, 王伟伟, 麦潋曦, 焦九阳, 谢舒乐, 林钊宇. 顺铂诱导舌鳞癌细胞发生上皮-间充质转化及获得肿瘤干细胞样特性的实验研究[J]. 中国口腔颌面外科杂志, 2020, 18(1): 10-16.
[14]	姚志涛, 安玮, 买买提吐逊·吐尔地. 50例唇腭裂患儿手术前、后血液生化指标及腭裂隙内骨再生评价[J]. 中国口腔颌面外科杂志, 2020, 18(1): 56-59.
[15]	阿力木江·苏甫尔, 比力克孜·玉素甫, 热娜古孜·阿不都米吉提. 新疆喀什地区非综合征型唇腭裂发病危险因素的病例对照研究[J]. 中国口腔颌面外科杂志, 2019, 17(5): 436-439.