利塞膦酸钠对骨质疏松大鼠牙槽骨Bcl-2、BAX表达的影响

doi:10.19438/j.cjoms.2018.05.004

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (5): 402-408.doi: 10.19438/j.cjoms.2018.05.004

利塞膦酸钠对骨质疏松大鼠牙槽骨Bcl-2、BAX表达的影响

魏立^1,2,*, 周琦^1,2,*, 江莉婷^1,3, 李宁¹, 高益鸣¹

1.上海交通大学医学院附属瑞金医院口腔科,上海 200025;
2.上海交通大学医学院附属瑞金医院上海市伤骨科研究所,上海 200025;
3.上海交通大学医学院附属第九人民医院·口腔医学院口腔修复科, 上海市口腔医学重点实验室,上海 200011;

收稿日期:2018-04-09 出版日期:2018-09-20 发布日期:2018-11-06
通讯作者: 高益鸣,E-mail：drgaoym@163.com
作者简介:魏立（1982-）,女,本科,主管技师,E-mail: hana_lily@163.com; 周琦（1980-）,女,本科,主管技师,E-mail：zhouqiqi931@hotmail.com。并列第一作者
基金资助:
上海市卫生和计划生育委员会科研项目（201640075）; 广慈卓越青年培养计划（GCQN-2017-B13）

Effects of risedronates on Bcl-2 and BAX expression in osteoporotic rat mandibular alveolar bone

WEI Li^1,2, ZHOU Qi^1,2, JIANG Li-ting^1,3, LI Ning¹, GAO Yi-ming¹

1.Department of Stomatology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine. Shanghai 200025;
2.Shanghai Institute of Traumatology and Orthopaedics. Shanghai 200025;
3.Department of Prosthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2018-04-09 Online:2018-09-20 Published:2018-11-06

摘要/Abstract

摘要： 目的: 观察利塞膦酸钠对骨质疏松大鼠下颌牙槽骨显微结构、抗凋亡因子Bcl-2、凋亡因子BAX表达以及骨细胞凋亡的影响。方法: 30只6月龄雌性SD大鼠,随机分为3 组（每组10只）,即Sham组（假手术组）、OVX组（接受双侧卵巢切除术）和RIS组（接受双侧卵巢切除术,然后给予利塞膦酸钠）;造模3个月后,取各组大鼠下颌骨进行micro-CT扫描,并通过TUNEL染色以及Bcl-2、BAX免疫组织化学染色检测下颌骨骨细胞的凋亡。采用SPSS 13.0软件包对组间数据进行比较。结果: 与Sham组相比,OVX组牙槽骨丧失和骨细胞凋亡明显增多;RIS组与OVX组相比,骨细胞凋亡显著减少,BV/TV显著增加,Bcl-2 表达增加,Bcl-2/BAX比值增加（P<0.05）。结论: 利塞磷酸钠增加下颌牙槽骨中Bcl-2的表达和Bcl-2/BAX的比值,部分逆转去卵巢骨质疏松大鼠的牙槽骨丧失,减少下颌骨骨细胞凋亡。

关键词: 卵巢切除术, 下颌牙槽骨, 二膦酸盐, 骨质疏松症, 骨细胞凋亡

Abstract: PURPOSE: This study was to investigate the impact of risedronates on mandibular alveolar bone microstructure, anti-apoptotic factor Bcl-2 and apoptotic factor BAX expression and osteocyte apoptosis in an ovariectomized rat model. METHODS: Thirty 6-month-old female SD rats were divided into 3 groups (ten in each group): Sham group, OVX group (bilateral ovariectomy) and RIS group (bilateral ovariectomy, followed by administration of risedronate). Three months later, the mandibles of all animals were harvested and assessed by micro-CT and histological analysis. Osteocyte apoptosis was observed by TUNEL staining and the expression of Bcl-2 and BAX was evaluated by immunohistochemical staining. The data were analyzed using SPSS 13.0 software package. RESULTS: Ovariectomy caused significant alveolar bone loss and greater osteocyte apoptosis in OVX group than in sham group. The systemic risedronate-treated group exhibited a significant increase in BV/TV and decrease in trabecular separation and osteocyte apoptosis, together with an increase in Bcl-2 expression and the ratio of Bcl-2/BAX, when compared with those of the OVX group (P<0.05). CONCLUSIONS: Systemic treatment of risedronates can increase the expression of Bcl-2 and the ratio of Bcl-2/BAX in mandibular alveolar bone. It partially reverses alveolar bone loss and suppresses osteocyte apoptosis in ovariectomized rats.

Key words: Ovariectomy, Mandibular alveolar bone, Bisphosphonates, Osteoporosis, Osteocyte apoptosis

中图分类号:

R782.1

魏立, 周琦, 江莉婷, 李宁, 高益鸣. 利塞膦酸钠对骨质疏松大鼠牙槽骨Bcl-2、BAX表达的影响[J]. 中国口腔颌面外科杂志, 2018, 16(5): 402-408.

WEI Li, ZHOU Qi, JIANG Li-ting, LI Ning, GAO Yi-ming. Effects of risedronates on Bcl-2 and BAX expression in osteoporotic rat mandibular alveolar bone[J]. China J Oral Maxillofac Surg, 2018, 16(5): 402-408.

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利塞膦酸钠对骨质疏松大鼠牙槽骨Bcl-2、BAX表达的影响

Effects of risedronates on Bcl-2 and BAX expression in osteoporotic rat mandibular alveolar bone

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