Bmi1对舌鳞癌侧群细胞迁移、侵袭和增殖能力的调控作用及机制探讨

doi:10.19438/j.cjoms.2018.02.002

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (2): 102-107.doi: 10.19438/j.cjoms.2018.02.002

Bmi1对舌鳞癌侧群细胞迁移、侵袭和增殖能力的调控作用及机制探讨

何倩婷¹, 汤磊乐², 孙菁菁¹, 卢志远¹, 王安训¹

1.中山大学附属第一医院口腔科,广东广州 510080;
2.中山大学附属第三医院心内科,广东广州 510000;

收稿日期:2017-09-18 修回日期:2017-11-27 出版日期:2018-03-20 发布日期:2018-04-08
通讯作者: 王安训,E-mail: anxunwang@yahoo.com
作者简介:何倩婷（1984-）,女,博士,E-mail: qiantinghe@hotmail.com
基金资助:
国家自然科学基金（81472523）; 广东省自然科学基金（2015A030313017）

Bmi1 mediated migration, invasion and proliferation of stem cells of tongue squamous cell carcinoma

HE Qian-ting¹, TANG Lei-le², SUN Jing-jing¹, LU Zhi-yuan¹, WANG An-xun¹

1.Department of Dentistry, First Affiliated Hospital of Sun Yat-sen University. Guangzhou 510080;
2.Department of Cardiology, Third Affiliated Hospital of Sun Yat-sen University. Guangzhou 510000, Guangdong Province, China;

Received:2017-09-18 Revised:2017-11-27 Online:2018-03-20 Published:2018-04-08

摘要/Abstract

摘要： 目的: 探讨Bmi1如何调控舌鳞癌侧群细胞的迁移、侵袭和增殖能力。 方法: 首先采用Hoechst33342法,利用流式细胞仪分选舌鳞癌细胞株UM1（高转移株）中的侧群细胞（side population cell, SP）和非侧群细胞（non-side population cell, Non-SP）。Transwell 实验检测沉默Bmi1后SP细胞的迁移、侵袭能力。球囊形成和平板克隆实验检测沉默Bmi1后SP细胞的球囊和克隆形成率;CCK8 实验检测沉默Bmi1后SP细胞的增殖能力。Western免疫印迹检测沉默Bmi1后SP细胞中侵袭、转移相关基因（SOD2、Slug）及干细胞标志物（ABCG2、Nanog）的表达水平。采用SPSS17.0软件包对数据进行统计学处理。 结果: 转染Bmi1 siRNA使SP细胞中Bmi1表达水平下调后,SP细胞的迁移和侵袭能力显著下降;球囊形成率和克隆形成率也显著低于对照组;增殖能力受到抑制;SOD2、Slug和干细胞标志物ABCG2和Nanog的表达水平显著下降。 结论: 沉默Bmi1可调控舌鳞癌侧群细胞的迁移、侵袭和增殖。

关键词: 舌鳞癌, 肿瘤干细胞, Bmi1, 迁移, 侵袭

Abstract: PURPOSE: To investigate the mechanism of Bmi1 mediating migration, invasion and proliferation of stem cells of tongue squamous cell carcinoma (TSCC). METHODS: SP (side population cell, SP) and non-SP (non-side population cell) cells were sorted by FCM using Hoechst33342 method. Transwell assay was performed to detect migration and invasion of SP cells after Bmi1 knockdown. Sphere forming assay was conducted to detect sphere forming rate of SP cells after Bmi1 knockdown. Colony forming assay was used to detect colony forming rate of SP cells after Bmi1 knockdown. CCK8 assay was used to detect proliferation of SP cells after Bmi1 knockdown. Western blot assay was used to detect expression of invasion and metastasis related genes (SOD2 and Slug) and stem cell markers (ABCG2 and Nanog) in SP cells after Bmi1 knockdown. The data was analyzed using SPSS 17.0 software package. RESULTS: After knockdown of Bmi1 in SP cells, migration and invasion were significantly inhibited compared to control siRNA transfection. SP cells transfected with Bmi1 siRNA displayed significantly decreased sphere formation and colony formation compared to the cells transfected with control siRNA. The proliferation of SP cells was significantly inhibited after transfection with Bmi1 siRNA. The expression of SOD2, Slug and stem cell markers (ABCG2, Nanog) in SP cells were significantly decreased after transfection with Bmi1 siRNA. CONCLUSIONS: Bmi1 knockdown inhibited migration, invasion and proliferation of cancer stem cells in TSCC.

Key words: Tongue squamous cell carcinoma, Cancer stem cell, Bmi1, Migration, Invasion

中图分类号:

R739.8

何倩婷, 汤磊乐, 孙菁菁, 卢志远, 王安训. Bmi1对舌鳞癌侧群细胞迁移、侵袭和增殖能力的调控作用及机制探讨[J]. 中国口腔颌面外科杂志, 2018, 16(2): 102-107.

HE Qian-ting, TANG Lei-le, SUN Jing-jing, LU Zhi-yuan, WANG An-xun. Bmi1 mediated migration, invasion and proliferation of stem cells of tongue squamous cell carcinoma[J]. China J Oral Maxillofac Surg, 2018, 16(2): 102-107.

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Bmi1对舌鳞癌侧群细胞迁移、侵袭和增殖能力的调控作用及机制探讨

Bmi1 mediated migration, invasion and proliferation of stem cells of tongue squamous cell carcinoma

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