低分子壳寡糖对LPS诱导的小鼠颅骨吸收的抑制作用

doi:10.19438/j.cjoms.2018.02.001

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (2): 97-101.doi: 10.19438/j.cjoms.2018.02.001

低分子壳寡糖对LPS诱导的小鼠颅骨吸收的抑制作用

郭克^1,*, 何佳嘉^{2, *}, 刘宗霖¹, 张耀升¹, 王文超¹, 王烨欣³, 徐伟峰^3#, 张善勇^3#

1.潍坊医学院口腔医学院,山东潍坊 261053;
2.南通市中西医结合医院（南通市口腔医院）,江苏南通 226001;
3.上海交通大学医学院附属第九人民医院·口腔医学院口腔外科,上海 200011;

收稿日期:2017-11-13 修回日期:2018-01-03 出版日期:2018-03-20 发布日期:2018-04-08
通讯作者: 张善勇,E-mail: zhangshanyong@126.com; 徐伟峰,E-mail: xwf19891215@163.com。^#共同通信作者
作者简介:郭克（1991-）,男,硕士,E-mail：guoke1366@126.com;何佳嘉（1988-）,男,E-mail：731744104@qq.com。<sup>*</sup>并列第一作者
基金资助:
国家自然科学基金（81371168、81671010）; 上海交通大学医学院转化医学创新基金（15ZH2007）; 上海市科学技术委员会生物医药支撑项目（16441908800）; 上海交通大学医学院科创项目（20160505）

Inhibition of chitosan oligosaccharide on skull resorption in mice induced by lipopolysaccharides

GUO Ke¹, HE Jia-jia², LIU Zong-lin¹, ZHANG Yao-sheng¹, WANG Wen-chao¹, WANG Ye-xin³, XU Wei-feng³, ZHANG Shan-yong³

1.College of Stomatology, Weifang Medical University. Weifang 261053, Shandong Province;
2.Stomatological Hospital of Nantong City. Nantong 226001, Jiangsu Province;
3.Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine. Shanghai 200011,China;

Received:2017-11-13 Revised:2018-01-03 Online:2018-03-20 Published:2018-04-08

摘要/Abstract

摘要： 目的: 研究低分子量壳寡糖（low-molecular chitosan oligosaccharide,LMCOS）对脂多糖（lipopolysaccharides,LPS）诱导的小鼠颅骨吸收模型的影响,评估其在小鼠颅骨吸收过程中的抑制作用。 方法: 将6周龄C57/BL6雄性小鼠随机分为 5组,即磷酸缓冲液（PBS）组、LPS组、LPS+0.005% LMCOS组、LPS+0.05% LMCOS组和LPS+0.5% LMCOS组;每组5只,LPS的刺激量为10 mg/kg。小鼠浅麻醉后,于颅盖骨皮下注射相应试剂,每只注射300 μL。每周小鼠头部皮下组织和颅骨骨膜之间注射试剂3次,2周后处死,取出颅盖骨进行Micro-CT扫描、H-E染色、TRAP 染色。采用SPSS21.0软件包对数据进行单因素方差分析,观察骨破坏面积、破骨细胞的形成等骨缺损表现。 结果: Micro-CT扫描、H-E染色、TRAP 染色发现,与PBS组相比,LPS组小鼠颅骨表面的骨破坏十分明显;加入不同浓度的LMCOS后,颅骨骨破坏面积及破骨细胞数目随LMCOS浓度的升高而不断减少。 结论: LMCOS具有抑制LPS诱导的小鼠颅骨骨吸收的作用,提示LMCOS对骨破坏性疾病可能具有一定的治疗作用。

关键词: 壳寡糖, 脂多糖, Micro-CT, TRAP, 骨吸收

Abstract: PURPOSE: To study the effect of low molecular weight chitosan (low-molecular chitosan oligosaccharide, LMCOS) on lipopolysaccharides (LPS) -induced bone resorption in mice. METHODS: Six-week c57/bl6 male mice were randomly divided into 5 groups: phosphate buffer saline (PBS) group, LPS group, LPS+0.005% LMCOS group, LPS+0.05% LMCOS group and LPS+0.5% LMCOS group. There were 5 mice in each group, LPS was used at 10 mg/kg. After mild anesthesia, 300 μL corresponding reagents were injected into the skull bone. The mice were injected 3 times a week and sacrificed 2 weeks later. The skull bone was removed for micro-CT scan, H-E staining, TRAP staining. SPSS21.0 software package was used for statistical analysis. Bone damage area, osteoclast formation was observed and recorded. RESULTS: After LMCOS was added to LPS-induced skull resorption, bone destruction area decreased obviously; and with the increase of LMCOS concentration, bone destruction area and number of osteoclasts decreased gradually. CONCLUSIONS: LMCOS can inhibit the damage of skull bone in LPS-induced mice, which may have a therapeutic effect on osteolytic diseases.

Key words: Chitosan oligosaccharide, Lipopolysaccharides, Micro-CT, TRAP, Bone resorption

中图分类号:

R786.2

郭克, 何佳嘉, 刘宗霖, 张耀升, 王文超, 王烨欣, 徐伟峰, 张善勇. 低分子壳寡糖对LPS诱导的小鼠颅骨吸收的抑制作用[J]. 中国口腔颌面外科杂志, 2018, 16(2): 97-101.

GUO Ke, HE Jia-jia, LIU Zong-lin, ZHANG Yao-sheng, WANG Wen-chao, WANG Ye-xin, XU Wei-feng, ZHANG Shan-yong. Inhibition of chitosan oligosaccharide on skull resorption in mice induced by lipopolysaccharides[J]. China J Oral Maxillofac Surg, 2018, 16(2): 97-101.

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低分子壳寡糖对LPS诱导的小鼠颅骨吸收的抑制作用

Inhibition of chitosan oligosaccharide on skull resorption in mice induced by lipopolysaccharides

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