兔上唇唇裂术后创口愈合过程中增生性瘢痕的变化趋势

doi:10.19438/j.cjoms.2017.06.003

中国口腔颌面外科杂志 ›› 2017, Vol. 15 ›› Issue (6): 493-497.doi: 10.19438/j.cjoms.2017.06.003

兔上唇唇裂术后创口愈合过程中增生性瘢痕的变化趋势

刘峰^*, 张岱尊^*, 肖文林, 薛令法, 许尧祥

青岛大学附属医院口腔医学中心,山东省教育厅口腔医学重点实验室,山东青岛 266003

收稿日期:2017-01-23 修回日期:2017-04-21 出版日期:2017-11-20 发布日期:2017-12-21
通讯作者: 肖文林,E-mail：wenlinxiao@sina.com
作者简介:刘峰（1991-）,女,硕士研究生,E-mail：13070869707@163.com;张岱尊（1975-）,女,硕士,E-mail：zhangdaizun@sina.com。*并列第一作者
基金资助:
山东省自然科学基金（ZR2015HM022）

Research on wound healing and variation of hypertrophic scar in rabbit after cleft lip surgery

LIU Feng, ZHANG Dai-zun, XIAO Wen-lin, XUE Ling-fa, XU Yao-xiang

Department of Stomatology, the Affiliated Hospital of Qingdao University; Key Laboratory of Oral Clinical Medicine of Shandong Province. Qingdao 266003, Shandong Province, China

Received:2017-01-23 Revised:2017-04-21 Online:2017-11-20 Published:2017-12-21

摘要/Abstract

摘要： 目的观察兔唇裂术后瘢痕增生的变化趋势,探讨唇裂术后瘢痕最大增生程度发生时间,为后续研究基因治疗增生性瘢痕最有效时机的选择提供依据。方法选用同一子代近交系纯种新西兰白兔40只,采用Millard唇裂修复术,制造出创伤愈合的瘢痕模型,分别在术后第2、3、4、5周进行肉眼观察和瘢痕体积测量,切取上唇瘢痕组织进行免疫组织化学石蜡切片染色（IHC-P）、肌成纤维细胞（MFB）计数,提取瘢痕组织中α平滑肌肌动蛋白（α-SMA）进行蛋白质免疫印迹实验。所得数据采用SPSS 18.0软件包进行t检验。结果肉眼观察,兔上唇唇裂术后创口愈合在不同时期有不同变化,术后第3、4周,瘢痕收缩较为明显,其中瘢痕体积、MFB细胞计数及α-SMA的表达与术后第2、5周相比,有显著差异（P<0.01）。结论唇裂术后瘢痕增生严重程度大致呈正态分布,α-SMA作为MFB的分化标志物,为在蛋白水平测定瘢痕严重程度提供了客观依据。

关键词: 兔, 唇裂, 增生性瘢痕, 肌成纤维细胞, α平滑肌肌动蛋白

Abstract: PURPOSE: To observe the hyperplasia trend of hypertrophic scar after cleft lip surgery in rabbits, and to explore the exact time of the largest degree of hypertrophic scar after cleft lip repair, which provided a theoretical basis for the study of the most effective time of gene therapy for hypertrophic scars. METHODS: Forty New Zealand white rabbits from the same offspring were used to make wound healing model in cleft lip Millard's procedure. The volumes were measured respectively and granulation tissues were observed visually at 2nd week, 3rd week, 4th week and 5th week after operation. The rabbits were sacrificed and scar tissues were harvested in the indicated time. Immunohistochemical paraffin section (IHC-P), myofibroblast (MFB) cell count and Western blot were performed to detect the expression level of α-smooth muscle actin (α-SMA) in the scar tissue. SPSS 18.0 software package was used for statistical analysis. RESULTS: The results showed that the scar contraction was significant at 3rd and 4th week, comparing to 2nd and 5th week after surgery. The degree of scar hyperplasia after surgery was approximately in normal distribution. CONCLUSIONS: MFBs were important cells in scar formation and α-SMA was an important marker of MFBs. The study provides an objective basis for determination of scar severity by measuring the protein expression of α-SMA.

Key words: Rabbit, Cleft lip, Hypertrophic scar, Myofibroblast, α-smooth muscle actin

中图分类号:

R782.2

刘峰, 张岱尊, 肖文林, 薛令法, 许尧祥. 兔上唇唇裂术后创口愈合过程中增生性瘢痕的变化趋势[J]. 中国口腔颌面外科杂志, 2017, 15(6): 493-497.

LIU Feng, ZHANG Dai-zun, XIAO Wen-lin, XUE Ling-fa, XU Yao-xiang. Research on wound healing and variation of hypertrophic scar in rabbit after cleft lip surgery[J]. China J Oral Maxillofac Surg, 2017, 15(6): 493-497.

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兔上唇唇裂术后创口愈合过程中增生性瘢痕的变化趋势

Research on wound healing and variation of hypertrophic scar in rabbit after cleft lip surgery

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