RNA 干扰TEAD基因对舌癌细胞系Tca8113 增殖和侵袭的影响

中国口腔颌面外科杂志 ›› 2014, Vol. 12 ›› Issue (2): 112-115.

RNA 干扰TEAD基因对舌癌细胞系Tca8113 增殖和侵袭的影响

徐贤寅, 柳杨, 於俊

无锡市人民医院口腔科,江苏无锡 214023

收稿日期:2013-07-15 修回日期:2013-09-12 出版日期:2014-03-10 发布日期:2014-11-06
通讯作者: 徐贤寅,E-mail：imaginal_line@sian.com
作者简介:徐贤寅(1974-),男,硕士,副主任医师
基金资助:
南京医科大学科技发展基金(2013NJMU158)

Effect of RNA interference of TEAD gene on the proliferation and invasion of Tca8113 cell line

XU Xian-yin, LIU Yang, YU Jun

Department of Stomatology, Wuxi People’s Hospital. Wuxi 214023, Jiangsu Province, China

Received:2013-07-15 Revised:2013-09-12 Online:2014-03-10 Published:2014-11-06
Supported by:
Science and Technology Development Fund of Nanjing Medical University(2013NJNU158)

摘要/Abstract

摘要： 目的：应用RNA 干扰技术抑制人舌癌细胞株Tca8113 中内源TEAD基因的水平, 观察TEAD基因对舌癌细胞生物学行为的影响。方法：构建针对TEAD基因特异性siRNA 真核表达载体,将其转染至Tca8113,采用RT-PCR法检测转染后的Tca8113 细胞中TEAD基因的表达,采用CCK8技术检测细胞的增殖情况,采用Transwell 法检测细胞的迁移情况。实验数据采用SPSS13.0软件包进行单因素方差分析。结果：siRNA 干扰Tca8113 细胞后,TEAD基因的表达水平显著下降(P<0.05),细胞生长缓慢,体外侵袭能力下降。结论：通过RNA干扰技术阻断TEAD的表达,可抑制Tca8113 细胞的生长、增殖、迁移,提示TEAD基因在舌癌的发生、发展过程中起着重要作用。

关键词: 舌癌, 细胞生物学行为, TEAD基因, RNA 干扰

Abstract: PURPOSE: To investigate the effect of TEAD gene on biological behavior of tongue squamous cell carcinoma by endogenous TEAD gene knockdown using RNA interference technique in the tongue carcinoma cell line Tca8113. METHODS: Specific iRNA of TEAD gene was established and transfected into the cell line Tca8113. The TEAD gene expression in Tca8113 was detected after transfection using RT-PCR. Cell proliferation and migration were observed by CCK8 and Transwell methods, respectively. The experimental data was statistically analyzed by One-Way ANOVA with SPSS13.0 software package. RESULTS: After siRNA interference, Tca8113 cell line showed significantly decreased expression of TEAD gene (P<0.05), lower rate of proliferation (P<0.05), and weakened invasion (P<0.05) in vitro. CONCLUSIONS: The inhibition of TEAD gene expression by RNAi can suppress the growth, proliferation and migration of Tca8113 cell line, indicating a pivot role of TEAD gene in the pathogenesis of tongue squamous cell carcinoma.

Key words: Tongue carcinoma, Biological behavior, TEAD gene, RNA interference

中图分类号:

R739.8

徐贤寅, 柳杨, 於俊. RNA 干扰TEAD基因对舌癌细胞系Tca8113 增殖和侵袭的影响[J]. 中国口腔颌面外科杂志, 2014, 12(2): 112-115.

XU Xian-yin, LIU Yang, YU Jun. Effect of RNA interference of TEAD gene on the proliferation and invasion of Tca8113 cell line[J]. China J Oral Maxillofac Surg, 2014, 12(2): 112-115.

参考文献

[1] Davidson BJ, Root WA, Trock BJ. Age and survival from squamous cell carcinoma of the oral tongue [J]. Head Neck, 2001, 23(4): 273-279.
[2] Allocati N, Di Ilio C, De Laurenzi V. p63/p73 in the control of cell cycle and cell death [J]. Exp Cell Res, 2012, 318(11): 1285-1290.
[3] Zheng HX, Cai YD, Wang YD, et al. Fas signaling promotes motility and metastasis through epithelial-mesenchymal transition in gastrointestinal cancer [J]. Oncogene, 2013, 32(9): 1183-1192.
[4] Tremblay AM, Camargo FD. Hippo signaling in mammalian stem cells [J]. Semin Cell Dev Biol, 2012, 23(7): 818-826.
[5] Karpowicz P, Perez J, Perrimon N. The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration [J]. Development, 2010, 137(24): 4135-4145.
[6] Cherrett C, Furutani-Seiki M, Bagby S. The Hippo pathway: key interaction and catalytic domains in organ growth control, stem cell self-renewal and tissue regeneration [J]. Essays Biochem, 2012, 53: 111-127.
[7] Pan D. The Hippo signaling pathway in development and cancer [J]. Dev Cell, 2010, 19(4): 491-505.
[8] Zhao B, Li L, Lei Q, et al. The Hippo-YAP pathway in organ size control and tumorigenesis: an updated version [J]. Genes Dev, 2010, 24(9): 862-874.
[9] Wisotzkey RG, Konikoff CE, Newfeld SJ. Hippo pathway phylogenetics predicts monoubiquitylation of Salvador and Merlin/nf2 [J]. PLoS One, 2012, 7(12): e51599.
[10] Zhao B, Ye X, Yu J, et al. TEAD mediates YAP-dependent gene induction and growth control [J]. Genes Dev, 2008, 22(14): 1962-1971.
[11] Landin Malt A, Cagliero J, Legent K, et al. Alteration of TEAD1 expression levels confers apoptotic resistance through the transcriptional up-regulation of Livin [J]. PLoS One, 2012, 7(9): e45498.
[12] Liu-Chittenden Y, Huang B, Shim JS, et al. Genetic and pharmacological disruption of the TEAD-YAP complex suppresses the oncogenic activity of YAP [J]. Genes Dev,2012, 26(12): 1300-1305.