兔舌癌哨位淋巴结C/Gd复合物间质磁共振造影的实验研究

doi:10.19438/j.cjoms.2019.02.002

中国口腔颌面外科杂志 ›› 2019, Vol. 17 ›› Issue (2): 106-111.doi: 10.19438/j.cjoms.2019.02.002

兔舌癌哨位淋巴结C/Gd复合物间质磁共振造影的实验研究

徐豪越^1,2, 刘世恩³, 朱海涛⁴, 宋凯¹, 冯元勇¹, 吴大熊^4,*, 尚伟^1,*

1.青岛大学附属医院口腔颌面外科,山东青岛 266003;
2.青岛大学口腔医学院,山东青岛 266003;
3.青岛大学附属医院放射科,山东青岛 2660033;
4.青岛科技大学材料科学与工程学院,山东青岛 266042

收稿日期:2018-08-20 修回日期:2018-12-25 出版日期:2019-03-20 发布日期:2019-04-12
通讯作者: 尚伟,E-mail：liweishang@126.com;吴大雄, E-mail：dxwu100@163.com。^*共同通信作者
作者简介:徐豪越（1989-）,男,在读硕士研究生,E-mail：xuhaoyu90@163.com
基金资助:
国家自然科学基金（51472134）; 青岛市市南区科技发展计划项目（2018-4-014-YY）

Assessment of cervical lymph nodes metastasis using interstitial MR lymphography with C/Gd nanocomposite in a rabbit tongue cancer model

XU Hao-yue^1,2, LIU Shi-en³, ZHU Hai-tao⁴, SONG Kai¹, FENG Yuan-yong¹, WU Da-xiong³, SHANG Wei¹

1. Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Qingdao University. Qingdao 266003;
2. School of Stomatology, Qingdao University. Qingdao 266003;
3. Department of Radiology, Affiliated Hospital of Qingdao University. Qingdao 266003;
4. College of Materials Science and Engineering, Qingdao University of Science and Technology. Qingdao 266042, Shandong Province, China

Received:2018-08-20 Revised:2018-12-25 Online:2019-03-20 Published:2019-04-12

摘要/Abstract

摘要： 目的:探讨新型显影剂C/Gd纳米复合物间质磁共振造影（IMRLG）诊断兔VX2舌癌模型颈部哨位淋巴结转移的应用价值。方法:将VX2癌组织块植入16只健康新西兰大白兔左侧舌缘。25 d后,于兔双侧舌缘黏膜下注入造影剂C/Gd纳米复合物0.25 mL。分别于注射前及注射后5、10、15、20、30、40 min及24 h进行磁共振淋巴成像。计算特定时间节点的信号强化率(E%),定位哨位淋巴结,观察淋巴系统。注射造影剂24 h后,于双侧舌下黏膜注射0.2 mL亚甲蓝,行活体淋巴染色,解剖颈部淋巴结,行组织病理学检查。采用SPSS19.0软件包对数据进行统计学分析。结果:成功建立兔VX2舌癌模型16只（16/16）,其中发生淋巴结转移16只（16/16）。注射造影剂后10 min,肿瘤侧与对照侧淋巴结磁共振成像的E%具有显著差异（P<0.05）。IMRLG判断转移哨位淋巴结18个,在亚甲蓝活体染色下,解剖出淋巴结32个,其中病理学检测转移淋巴结17个,IMRLG的准确率为94.45%（17/18）,两者诊断SLN转移无统计学差异（P>0.05）。结论:应用C/Gd纳米复合物行IMRLG,可较准确地判断兔舌癌哨位淋巴结状况,为判断舌癌淋巴结转移提供了新的思路,有潜在应用价值。

关键词: 舌癌, 纳米碳, MR, 间质淋巴造影, 哨位淋巴结

Abstract: PURPOSE: To investigate the value of a novel imaging agent C/Gd nanocomposite interstitial magnetic resonance lymphography (IMRLG) in the diagnosis of cervical sentinel lymph node metastasis in a rabbit model of VX2 tongue cancer. METHODS: VX2 cancer tissue blocks were implanted into the left ventral submucosa of tongues in 16 healthy New Zealand white rabbits. After 25 days of implanting VX2, 0.2 mL of contrast agent C/Gd nanocomposite were injected into bilateral submucosa of rabbits on both sides. MR lymphography was performed at 5 min, 10 min, 15 min, 20 min, 30 min, 40 min and 24 h after injection. The signal enhancement rate (E%) was calculated, the sentinel lymph nodes was located and the characteristics of the lymphatic system was observed. After 24 hours of injecting contrast agent, 0.2 mL of methylene blue were injected into the bilateral submucosa for lymph staining. Subsequently, the cervical lymph nodes were dissected and histopathological examinations were performed. SPSS19.0 software package was used to analyze the data. RESULTS: Sixteen rabbit models with tongue transplanted tumor were successfully established. The cervical lymph node metastases were found in 16 rabbits. E% had significant difference between the tumor side and the control side ten minutes after injection of the contrast agent (P<0.05). IMRLG showed 18 metastatic nodes. Pathological examination indicated that 17/18 were positive, yielding a success rate of 94.45% (17/18). There was no significant difference between the two methods for diagnosing SLN metastasis (P>0.05). CONCLUSIONS: IMRLG by C/Gd nanocomplexes can be used to accurately determine the status of sentinel lymph node, which effectively provides a new way for detecting metastasis of lymph node of of tongue carcinoma.

Key words: Tongue cancer, Nano-carbon, MR, Interstitial lymphography, Sentinel lymph node

中图分类号:

R782

徐豪越, 刘世恩, 朱海涛, 宋凯, 冯元勇, 吴大熊, 尚伟. 兔舌癌哨位淋巴结C/Gd复合物间质磁共振造影的实验研究[J]. 中国口腔颌面外科杂志, 2019, 17(2): 106-111.

XU Hao-yue, LIU Shi-en, ZHU Hai-tao, SONG Kai, FENG Yuan-yong, WU Da-xiong, SHANG Wei. Assessment of cervical lymph nodes metastasis using interstitial MR lymphography with C/Gd nanocomposite in a rabbit tongue cancer model[J]. China J Oral Maxillofac Surg, 2019, 17(2): 106-111.

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兔舌癌哨位淋巴结C/Gd复合物间质磁共振造影的实验研究

Assessment of cervical lymph nodes metastasis using interstitial MR lymphography with C/Gd nanocomposite in a rabbit tongue cancer model

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