NLK与PTEN在头颈部鳞癌中的表达及两者的相关性分析

doi:10.19438/j.cjoms.2025.04.005

中国口腔颌面外科杂志 ›› 2025, Vol. 23 ›› Issue (4): 340-346.doi: 10.19438/j.cjoms.2025.04.005

NLK与PTEN在头颈部鳞癌中的表达及两者的相关性分析

武国浩¹, 付波², 张迪², 李君², 许凯³, 李克义^3,*, 张冬燕^2,*

1.山东第二医科大学口腔医学院,山东潍坊 261053;
2.聊城市人民医院精准生物医学重点实验室,山东聊城 252000;
3.聊城市人民医院口腔颌面外科,山东聊城 252000

收稿日期:2025-02-11 修回日期:2025-03-17 出版日期:2025-07-20 发布日期:2025-08-04
通讯作者: 李克义,E-mail：lkyxyw@163.com;张冬燕,E-mail：dongyan_1126@126.com。^*共同通信作者
作者简介:武国浩（1997-）,男,硕士研究生,E-mail：15662588380@163.com
基金资助:
山东省医药卫生科技发展计划项目（2019WS115）;聊城市重点研发计划项目（2023YD18）

The expression and correlation of NLK and PTEN in head and neck squamous cell carcinoma

Wu Guohao¹, Fu Bo², Zhang Di², Li Jun², Xu Kai³, Li Keyi³, Zhang Dongyan²

1. School of Stomatology, Shandong Second Medical University. Weifang 261053;
2. Key Laboratory of Precision Biomedicine, Liaocheng People's Hospital. Liaocheng 252000;
3. Department of Oral and Maxillofacial Surgery, Liaocheng People's Hospital. Liaocheng 252000, Shandong Province, China

Received:2025-02-11 Revised:2025-03-17 Online:2025-07-20 Published:2025-08-04

摘要/Abstract

摘要： 目的：探讨Nemo样激酶（Nemo-like kinase,NLK）以及磷酸酶和张力蛋白同源物（phosphatase and tensin homolog deleted on chromosome ten,PTEN）在头颈部鳞癌（head and neck squamous cell carcinoma,HNSCC）中的表达及与临床病理特征的关系,并初步探讨NLK与PTEN之间的相关性。方法：从癌症基因组图谱（The Cancer Genome Atlas,TCGA）数据库提取HNSCC患者相关基因表达和临床特征数据,使用R语言和Bioconductor包进行数据处理,应用在线平台进行数据分析。选取32例HNSCC患者标本,应用免疫组织化学方法检测NLK和PTEN的表达,并验证两者间的相关性。结果：通过对TCGA数据库收集的520例病例的分析发现,NLK和PTEN在HNSCC中有显著的表达改变,并且在性别、临床分期、T分期、N分期及HPV感染队列中存在显著差异。在不同部位的HNSCC中,NLK和PTEN也存在表达差异。免疫组织化学染色结果与TCGA数据分析均显示,NLK和PTEN的表达呈负相关。结论：NLK和PTEN在HNSCC不同临床病理特征队列中存在表达差异,且两者表达呈负相关。

关键词: NLK, PTEN, 头颈部鳞癌, 临床病理特征

Abstract: PURPOSE: To examine the expression levels of Nemo-like kinase (NLK) and phosphatase and tensin homolog deleted on chromosome ten (PTEN) in head and neck squamous cell carcinoma (HNSCC), and to assess the association between their expression and the clinicopathological characteristics of HNSCC patients, as well as the interrelationship between NLK and PTEN. METHODS: Gene expression data and clinical information for HNSCC patients were sourced from The Cancer Genome Atlas (TCGA) database. Data processing was performed using the R programming language and Bioconductor packages, with data analysis facilitated by online platforms. Immunohistochemistry was employed to detect NLK and PTEN expression in 32 HNSCC specimens to validate their correlation. RESULTS: The analysis of 520 cases collected from TCGA database showed that the expression of NLK and PTEN in HNSCC was significantly changed, and there were significant differences in gender, clinical stage, T stage, N stage and HPV infection cohort. In HNSCC of different sites, the expression of NLK and PTEN was also different. In addition, both the immunohistochemical results and the TCGA data analysis showed a negative correlation in the expression levels of NLK and PTEN. CONCLUSIONS: NLK and PTEN exhibit differential expression across HNSCC cohorts with distinct clinicopathological features, and their expression levels are inversely correlated.

Key words: NLK, PTEN, Head and neck squamous cell carcinoma, Clinicopathological feature

中图分类号:

R739.8

武国浩, 付波, 张迪, 李君, 许凯, 李克义, 张冬燕. NLK与PTEN在头颈部鳞癌中的表达及两者的相关性分析[J]. 中国口腔颌面外科杂志, 2025, 23(4): 340-346.

Wu Guohao, Fu Bo, Zhang Di, Li Jun, Xu Kai, Li Keyi, Zhang Dongyan. The expression and correlation of NLK and PTEN in head and neck squamous cell carcinoma[J]. China J Oral Maxillofac Surg, 2025, 23(4): 340-346.

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NLK与PTEN在头颈部鳞癌中的表达及两者的相关性分析

The expression and correlation of NLK and PTEN in head and neck squamous cell carcinoma

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