胎盘蛋白11在口腔鳞癌中的表达及临床意义

doi:10.19438/j.cjoms.2025.04.006

摘要/Abstract

摘要： 目的：探讨胎盘蛋白11（ENDOU）在口腔鳞癌（OSCC）中的表达及临床意义。方法：利用TCGA数据库对比分析ENDOU在肿瘤组织和正常组织中的表达,分析ENDOU表达与临床参数的相关性。随后,通过免疫组织化学染色检测20例原发OSCC及对应癌旁组织中ENDOU的表达。构建ENDOU过表达的人OSCC细胞系SCC-25,通过CCK-8实验、流式细胞凋亡实验及Transwell细胞侵袭实验评估ENDOU对OSCC细胞增殖、凋亡和侵袭的影响。结果：生物信息分析结果显示,在OSCC中,ENDOU基因的表达显著低于癌旁组织（P<0.05）;ENDOU基因在原发肿瘤的不同T分期以及临床分期中有统计学差异（P<0.05）,颈淋巴结转移组显著低于无淋巴结转移组（P<0.05）。免疫组织化学染色结果显示,OSCC组织中ENDOU的表达水平显著低于癌旁组织（P<0.05）;过表达ENDOU能够在体外抑制OSCC细胞的增殖和迁移。结论：OSCC中ENDOU表达下调,可能在肿瘤的形成和发展中扮演着重要角色,有望成为OSCC的生物标志物。

关键词: 胎盘蛋白11, 口腔鳞癌, 增殖, 侵袭, 凋亡

Abstract: PURPOSE: To investigate the expression and clinical significance of placental protein 11 (ENDOU) in oral squamous cell carcinoma(OSCC). METHODS: The expression of ENDOU in tumor tissues and normal tissues was comparatively analyzed through the TCGA database, and the correlation between ENDOU expression and clinical parameters was explored. Subsequently, the expression of ENDOU in 20 cases of primary OSCC and corresponding adjacent tissues was detected by immunohistochemical staining. The ENDOU overexpressing human OSCC cell line SCC-25 was constructed. Furthermore, the effects of ENDOU on the proliferation, apoptosis and invasion of OSCC cells were evaluated by CCK-8 assay, flow cytometry apoptosis assay, and Transwell cell invasion assay. RESULTS: Bioanalytical results indicated that the expression of the ENDOU gene in OSCC was significantly lower than that in adjacent tissues(P<0.05); there was significant difference in the expression of the ENDOU gene among different T stages and clinical stages of primary tumors(P<0.05), and the expression was significantly lower in cervical lymph node metastasis group than in the non-metastasis group(P<0.05). Immunohistochemical experiments showed that the expression level of ENDOU in OSCC tissues was significantly lower than that in adjacent tissues (P<0.05); overexpression of ENDOU could inhibit the proliferation and migration of oral squamous cell carcinoma cells in vitro. CONCLUSIONS: Downregulation of EDNOU expression in OSCC may play an important role in tumor formation and development, and is expected to serve as a tumor biomarker for OSCC.

Key words: Placental protein 11, Oral squamous cell carcinoma, Proliferation, Invasion, Apoptosis

中图分类号:

R739.8

阿拉发提·何亚斯丁, 安玮, 加娜尔·吐尔根别克, 金雪梅, 郭莎, 姚志涛. 胎盘蛋白11在口腔鳞癌中的表达及临床意义[J]. 中国口腔颌面外科杂志, 2025, 23(4): 347-354.

Alafati Heyasiding, An Wei, Janaer Tuergenbieke, Jin Xuemei, Guo Sha, Yao Zhitao. Expression of placental protein 11 in oral squamous cell carcinoma and its clinical significance[J]. China J Oral Maxillofac Surg, 2025, 23(4): 347-354.

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