NLRP1在头颈部鳞癌中的表达及预后意义

doi:10.19438/j.cjoms.2022.05.003

中国口腔颌面外科杂志 ›› 2022, Vol. 20 ›› Issue (5): 429-436.doi: 10.19438/j.cjoms.2022.05.003

NLRP1在头颈部鳞癌中的表达及预后意义

杨慧丽^1,2, 王宇帆², 王锋², 沈月洪^2,*, 杨宏宇^1,2,*

1.北京大学口腔医学院·口腔医院综合二科,国家口腔医学中心,国家口腔疾病临床医学研究中心,口腔生物材料和数字诊疗装备国家工程研究中心,北京 100081;
2.北京大学深圳医院口腔医学中心口腔颌面外科,广东省高水平临床重点专科,广东省口腔疾病诊疗技术工程技术研究中心,广东深圳 518036

收稿日期:2022-01-11 修回日期:2022-04-19 出版日期:2022-09-20 发布日期:2022-09-20
通讯作者: 沈月洪,E-mail: yuehongshen@hotmail.com;杨宏宇,E-mail: hyyang192@hotmail.com。^*共同通信作者
作者简介:杨慧丽（1996-）,女,硕士,E-mail: yanghl0809@163.com
基金资助:
广东省基础与应用基础研究基金（2019A1515011911）; 深圳市科技创新委员会基础研究重点项目基金（JCYJ20200109140208058）

Comprehensive co-expression analysis revealed NLRP1 as a prognostic immune-associated gene in head and neck squamous cell carcinoma

YANG Hui-li^1,2, WANG Yu-fan², WANG Feng², SHEN Yue-hong², YANG Hong-yu^1,2

1. Department of General Dentistry II, Peking University School and Hospital of Stomatology; National Center of Stomatology; National Clinical Research Center for Oral Diseases; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices. Beijing 100081;
2. Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital; Guangdong Provincial High-Level Clinical Key Specialty; Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment. Shenzhen 518036, Guangdong Province, China

Received:2022-01-11 Revised:2022-04-19 Online:2022-09-20 Published:2022-09-20

摘要/Abstract

摘要： 目的: 研究含热蛋白结构域的NOD样受体蛋白1（NLRP1）在头颈部鳞癌（HNSCC）中的表达、预后价值及免疫相关性。方法: 通过基于癌症基因组图谱 (TCGA) 数据库的多个在线网站,分析NLRP1表达及在HNSCC中的预后价值,并通过GO、KEGG分析,明确其参与的生物过程及信号通路。结果: NLRP1在HNSCC中上调,NLRP1的高表达与较长的总生存期呈正相关。功能网络分析及共表达分析表明,NLRP1与先天免疫及炎症等相关,在多个免疫相关的信号通路中发挥作用。此外,在HNSCC中,NLRP1的表达与中性粒细胞、CD4⁺T细胞、树突状细胞的浸润呈正相关,NLRP1与HNSCC中的多种免疫标志物相关。结论: NLRP1在HNSCC中高表达,NLRP1的高表达可改善预后,可作为HNSCC的潜在预后标志物。NLRP1在先天免疫反应和炎症反应中发挥作用,NLRP1高表达与HNSCC中CD4⁺ T细胞、中粒细胞、树突状细胞的浸润程度呈正相关。

关键词: NLRP1, 头颈部鳞癌, 生物标志物, 预后, 免疫浸润

Abstract: PURPOSE: To explore the prognostic value and mechanism of NLR family, pyrin domain containing 1 (NLRP1) in head and neck squamous cell carcinoma(HNSCC). METHODS: This study performed bioinformatics analysis on HNSCC data from The Cancer Genome Atlas (TCGA), and jointly analyzed with online databases such as TIMER, TCGAportal, Kaplan-Meier Plotter, LinkedOmics, GeneMANIA, Metascape, TISIDB and miRDB. RESULTS: NLRP1 was up-regulated in HNSCC. Kaplan-Meier survival analysis indicated that patients with high NLRP1 expression had a better prognosis than those with low expression. Furthermore, NLRP1 was concentrated in multiple immune-associated signaling pathways and the expression of NLRP1 was associated with the infiltration of CD4⁺T cells, neutrophils and dendritic cells. CONCLUSIONS: Our findings confirmed that NLRP1 is highly expressed in HNSCC. The high expression of NLRP1 was significantly related to prognosis and the infiltration degree of CD4⁺ T cells, neutrophils and dendritic cells in HNSCC.

Key words: NLRP1, Head and neck squamous cell carcinoma, Biomarkers, Prognosis, Immune infiltrate

中图分类号:

R739.8

杨慧丽, 王宇帆, 王锋, 沈月洪, 杨宏宇. NLRP1在头颈部鳞癌中的表达及预后意义[J]. 中国口腔颌面外科杂志, 2022, 20(5): 429-436.

YANG Hui-li, WANG Yu-fan, WANG Feng, SHEN Yue-hong, YANG Hong-yu. Comprehensive co-expression analysis revealed NLRP1 as a prognostic immune-associated gene in head and neck squamous cell carcinoma[J]. China J Oral Maxillofac Surg, 2022, 20(5): 429-436.

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NLRP1在头颈部鳞癌中的表达及预后意义

Comprehensive co-expression analysis revealed NLRP1 as a prognostic immune-associated gene in head and neck squamous cell carcinoma

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