BMP-2联合雷奈酸锶对大鼠骨髓间充质干细胞成骨分化的影响

doi:10.19438/j.cjoms.2024.05.003

中国口腔颌面外科杂志 ›› 2024, Vol. 22 ›› Issue (5): 430-434.doi: 10.19438/j.cjoms.2024.05.003

BMP-2联合雷奈酸锶对大鼠骨髓间充质干细胞成骨分化的影响

袁润林¹, 李亚明²

1.乐山老年病专科医院口腔科门诊,四川乐山 614000;
2.成都市第二人民医院口腔科, 四川成都 610000

收稿日期:2023-11-15 修回日期:2024-07-08 出版日期:2024-09-20 发布日期:2024-09-29
通讯作者: 李亚明,E-mail: 18884864463@163.com
作者简介:袁润林（1976-）,男,学士,主治医师,E-mail: 18908136330@189.com

Effect of BMP-2 combined with strontium ranelate on osteogenic differentiation of rat bone marrow mesenchymal stem cells

YUAN Run-lin¹, LI Ya-ming²

1. Outpatient Stomatology Clinic, Leshan Geriatric Specialised Hospital. Leshan 614000, Sichuan Province;
2. Department of Stomatology, Chengdu Second People's Hospital. Chengdu 610000, Sichuan Province, China

Received:2023-11-15 Revised:2024-07-08 Online:2024-09-20 Published:2024-09-29

摘要/Abstract

摘要： 目的: 研究骨形态发生蛋白2（bone morphogenetic protein-2,BMP-2）联合雷奈酸锶（strontium ranelate,Sr）对大鼠骨髓间充质干细胞（bone mesenchymal stem cells,BMSCs）成骨分化的影响,并探讨其相关机制。方法: 取对数期大鼠BMSCs,随机分为对照组（不采用任何诱导液处理）、BMP-2组（0.1 mg/L BMP处理）、Sr组（5 mmol/L Sr处理）、BMP-2+Sr组（采用0.1 mg/L BMP处理6天后换液,再加入5 mmol/L Sr处理）。利用MTT实验检测BMSCs增殖情况,硝基苯磷酸盐法检测碱性磷酸酶（alkaline phosphatase,ALP）活性,茜素红染色观察矿化情况,Western免疫印迹法检测TGF-β1、Smad2、p-Smad2及Runx2蛋白表达。采用SPSS 24.0软件包对数据进行统计学分析。结果: 与对照组相比,BMP-2组、Sr组、BMP-2+Sr组ALP活性增强,MTT实验吸光度值、矿化面积百分比及TGF-β1、Runx2蛋白表达量,p-Smad2/Smad2水平显著升高（P<0.05）。与BMP-2组、Sr组相比,BMP-2+Sr组ALP活性增强,MTT实验吸光度值、矿化面积百分比及TGF-β1、Runx2蛋白表达量,p-Smad2/Smad2水平显著升高（P<0.05）。结论: BMP-2联合Sr可促进大鼠BMSCs增殖及成骨分化,其作用机制可能与激活TGF-β1/Smad信号通路有关。

关键词: 大鼠, 骨髓间充质干细胞, 成骨分化, 骨形态发生蛋白2, 雷奈酸锶

Abstract: PURPOSE: To study the effect of bone morphogenetic protein-2(BMP-2) combined with strontium ranelate (Sr) on osteogenic differentiation of rat bone mesenchymal stem cells(BMSCs), and explore the related mechanisms. METHODS: Log-phase BMSCs were taken and randomly divided into control group (without any induction solution treatment), BMP-2 group (0.1 mg/L BMP treatment), Sr group (5 mmol/L Sr treatment), BMP-2+Sr group (after treatment with 0.1 mg/L BMP for 6 days, changed the solution and added 5 mmol/L Sr for treatment). Cell proliferation was detected by MTT experiment. Alkaline phosphatase(ALP) activity was detected by nitrophenyl phosphate method. Mineralization was observed by alizarin red staining. Protein expression of transforming growth factor β1(TGF-β1), Smad2, p-Smad2, and Runt-related transcription factor 2 (Runx2) were detected by Western blot. SPSS 24.0 software package was used for data analysis. RESULTS: Compared with the control group, the ALP activity of BMP-2 group, Sr group, and BMP-2+Sr group were enhanced, of which MTT experimental absorbance(A) value, the percentage of mineralized area and the expression of TGF-β1 and Runx2 protein, p-Smad2/Smad2 were significntly increased(P<0.05). Compared with BMP-2 group and Sr group, the ALP activity of the BMP-2+Sr group were enhanced, of which MTT experimental A value, the percentage of mineralized area and the expression of TGF-β1 and Runx2 protein, p-Smad2/Smad2 were significantly increased(P<0.05). CONCLUSIONS: BMP-2 combined with Sr can promote the proliferation and osteogenic differentiation of BMSCs in rats, the mechanism of which may be related to the activation of TGF-β1/Smad signaling pathway.

Key words: Rat, Bone mesenchymal stem cells, Osteogenic differentiation, Bone morphogenetic protein-2, Strontium ranelate

中图分类号:

R336

袁润林, 李亚明. BMP-2联合雷奈酸锶对大鼠骨髓间充质干细胞成骨分化的影响[J]. 中国口腔颌面外科杂志, 2024, 22(5): 430-434.

YUAN Run-lin, LI Ya-ming. Effect of BMP-2 combined with strontium ranelate on osteogenic differentiation of rat bone marrow mesenchymal stem cells[J]. China J Oral Maxillofac Surg, 2024, 22(5): 430-434.

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BMP-2联合雷奈酸锶对大鼠骨髓间充质干细胞成骨分化的影响

Effect of BMP-2 combined with strontium ranelate on osteogenic differentiation of rat bone marrow mesenchymal stem cells

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